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Johanne Dodon, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana
The aim of the study was to evaluate the effect of the The Trivedi Effect®- Consciousness Energy Healing Treatment based new proprietary herbomineral formulation and cell medium (DMEM) on various skin health parameters using HFF-1, HaCaT, and B16-F10 cell lines. It contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts each, one part of each was coded as untreated (UT), while the second part of each was treated with the Biofield Treatment by Johanne Dodon and denoted as the Biofield Energy Treated (BT) test items. MTT assay result showed that the test formulation was safe and nontoxic up to 80 µg/mL concentration. The fibroblast cell proliferation assay using BrdU method showed a significant increased cell proliferation at 2.13, 8.75, and 17.53 µg/mL by 3.90%, 14.68% and 29.54%, respectively in the BT-DMEM + BT-Test formulation group compared to the untreated group. The collagen data showed an increased synthesis by 7.42% and 3.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Similarly, the elastin synthesis was increased by 19.8% and 25.8% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL with respect to the untreated group. However, the Biofield Energy Treated test formulation showed an increased level of hyaluronic acid by 1.7% in UT-DMEM + BT-Test formulation group compared to the untreated group. Melanin synthesis inhibition was observed by 15.21% and 11.43% in UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation groups, respectively at 0.125 µg/mL compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling effects showed an improved cell viability by 7.65% at 1.25 µg/mL in the BT-DMEM + BT-Test formulation experimental group compared with the untreated test group. In addition, the Biofield Energy Treated Test formulation/DMEM showed a significant wound healing activity and high cellular migration of fibroblast and keratinocytes in HFF-1 and HaCaT cells, respectively. Overall, the data suggested that Biofield Treated formulation/DMEM could be helpful for skin healing, improvement the skin health, elasticity, and tightness, as well as, improving skin health issues such as wrinkling, pigmentation disorders, redness, swelling, skin cancer, aging, rashes, and skin sagging.