Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 162 after revival (Study I). The revived sample of Gr. IIB was retreated on day 162 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively after their sub-culturing. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The antimicrobial susceptibility and minimum inhibitory concentration were significantly improved by 71.43%, out of twenty-eight and 56.25%, out of thirty-two, respectively in the treated cells of P. rettgeri as compared to the control. The biochemical reactions also showed the significant (60.61%) alteration in the treated sample with respect to control. The biotype numbers were substantially changed in all the treated groups as compared to the control. Moreover, the organism was changed as Proteus mirabilis in all the treated groups except in Gr. IIA, as compared to the control. These results suggested that biofield treatment has a significant impact on P. rettgeri in lyophilized as well as revived state.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Complementary and alternative medicine (CAM) has become increasingly popular and reported for countless benefits in biomedical health care systems. The study assessed the potential impact of The Trivedi Effect® (biofield energy) on Shigella sonnei for changes in antimicrobial sensitivity, biochemical study, and biotype number using MicroScan Walk-Away® system. The cells were obtained from MicroBioLogics Inc., USA bearing the American Type Culture Collection (ATCC 9290) number, and divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was subjected to Mr. Trivedi’s biofield energy treatment and further subdivided into two sub-groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 160 (Study I). The Gr. IIB sample was retreated on day 160 (Study II), and was divided into three separate tubes as first, second and third tube, which were analyzed on day 5, 10 and 15, respectively. Results showed that 35% (7 out of 20) antimicrobials were reported with improved sensitivity profile. Moreover, the minimum inhibitory concentration study showed that 56.25% (18 out of 32) tested antimicrobials were reported with decreased concentration by two to four-fold as compared with the control after biofield treatment. The effect was further analyzed and sustained in the biochemical study, where 57.57% (19 out of 33) tested biochemicals showed altered reaction pattern as compared with the control. The biotype study showed an alteration in the biotype number in all the experimental treated groups as compared to the control. Encouraging results suggests that bioenergy healing treatment as an integrative medicine against S. sonnei would be a better and safe treatment approach in near future.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Gunin Saikia, Snehasis Jana

The aim of the present study was to evaluate the impact of biofield energy treatment on the thermal, spectroscopic, and chemical properties of benzophenone. The study was done using various analytical methods such as gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-Vis) spectroscopy. The benzophenone sample was divided into two parts, one part was subjected to Mr. Trivedi’s biofield energy treatment, called as treated and the other part was remained as untreated, called as control. Mass spectra showed the molecular ion peak at m/z = 182 in control and all the treated benzophenone samples with different intensities (treated samples further divided in to three parts, T1, T2, and T3 for GC-MS study). The isotopic abundance ratio of 2H/1H, 13C/12C (PM+1)/PM and in treated sample was decreased by 44.87% in T2 and slightly increased upto 5.79% in case of T1 as compared to the control [where, PM- primary molecule, (PM+1)- isotopic molecule either for 13C or 2H]. Moreover, isotopic abundance ratio of 18O/16O (PM+2)/PM in the treated sample was increased up to 22.64% in T3. The retention time of treated benzophenone was slightly increased (0.88 min) as compared to the control in HPLC chromatogram. The DSC data exhibited that the heat of degradation of treated benzophenone was increased by 674.16% as compared to the control. While, C=O stretching frequency of treated sample was shifted by 6 cm-1 to low energy region in FT-IR spectroscopy. Further, the UV-Vis spectra of control sample showed characteristic absorption peaks at 210 nm and 257 nm that was blue shifted to 205 nm and 252 nm, respectively in the treated sample. These results suggested that biofield treatment has significantly altered the thermal, spectroscopic, and chemical properties of benzophenone, which could make them more useful as reaction intermediate in industrial applications.

Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

Biphenyl is used as an intermediate for synthesis of various pharmaceutical compounds. The objective of present research was to investigate the influence of biofield treatment on physical, spectroscopic and thermal properties of biphenyl. The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated biphenyl were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, Ultraviolet-visible (UV-Vis) spectroscopy and surface area analysis. The treated biphenyl showed decrease in intensity of XRD peaks as compared to control. Additionally, crystallite size was decreased in treated biphenyl by 16.82% with respect to control. The treated biphenyl (72.66ºC) showed increase in melting temperature as compared to control biphenyl (70.52ºC). However, the latent heat of fusion (∆H) of treated biphenyl was substantially changed by 18.75% as compared to control. Additionally, the treated biphenyl (155.14ºC) showed alteration in maximum thermal decomposition temperature (Tmax) as compared to control sample (160.97ºC). This showed the alteration in thermal stability of treated biphenyl as compared to control. Spectroscopic analysis (FT-IR and UV-visible) showed no alteration in chemical nature of treated biphenyl with respect to control. Surface area analysis through Brunauer-Emmett-Teller analysis (BET) analyzer showed significant alteration in surface area as compared to control. Overall, the result demonstrated that biofield has substantially affected the physical and thermal nature of biphenyl.