Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Sodium selenate is a common ingredient in nutraceuticals/pharmaceuticals as a source of selenium. The objective of the current study was to evaluate the impact of The Trivedi Effect® - Energy of Consciousness (Biofield Energy Healing) on the physicochemical, spectral, and thermal properties of The Trivedi Effect® Treated sodium selenate using various analytical methods such as PXRD, PSD, FT-IR, UV-vis, DSC, and TGA. Sodium selenate was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® Treatment remotely from eighteen renowned Biofield Energy Healers. The PXRD analysis of the treated sample showed the significant alteration of the crystallite size in the range of -16.63% to 42.9% compared with the control sample. However, the average crystallite size of the treated sodium selenate was increased by 1.01% compared with the control sample. The particle sizes d10, d50, and d90 values of the treated sample were significantly decreased by 6.14%, 16.56%, and 18.82%, respectively compared with the control sample. Consequently, the surface area of the treated sample was significantly increased by 10.52% compared with the control sample. Both control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 888 and 887 cm-1, respectively due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of the control and treated sodium selenate were at 204.8 and 204.9 nm, respectively. The TGA of the control and treated samples exhibited two steps of thermal degradation, and the total weight loss was slightly increased by 1.16% in the treated sample compared with the control sample. The DSC thermograms showed the latent heat of fusion (ΔH) was significantly elevated by 6.37% in the treated sample compared with the control sample. The current study revealed that The Trivedi Effect® - Energy of Consciousness (Biofield Energy Healing) might generate a new polymorphic form of sodium selenate which could be more soluble, thermally more stable, and higher absorption rate compared with the untreated sample. Hence, The Trivedi Effect® treated sodium selenate would be very useful to design the various forms of nutraceuticals and/or pharmaceutical formulation which might be providing a better therapeutic response against heart disease (i. e. Atherosclerosis), viral diseases (i. e. Hepatitis), infectious diseases (i. e. Dermatitis, Psoriasis), Parkinson’s Disease, Alzheimer’s Disease, etc.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on phytoconstituents present in the ashwagandha root extract using GC-MS and NMR. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The GC-MS data indicated that the peak height and peak area of The Trivedi Effect® treated sample were found to be altered compared with the control sample. The peak height of the phytoconstituents present in the treated ashwagandha sample was altered significantly in the range of -8.32% to 89.25% compared with the control sample. Similarly, the peak area of the treated sample was altered significantly in the range of - 4.28% to 216.30% compared with the control sample. Overall, the change in the peak area% of the treated sample was significantly altered in the range of -18.29% to 170.18% compared with the control sample. The GC-MS and NMR analysis results identified the presence of withanolides such as glyco-withanolides, alkaloids, and sugars in the root extract in both the sample. The peak area of 2,3,4,5-tetrahydropyridazine (1), methyl ethyl sulfoxide (2), 5,6-dihydro-2-methyl-4(H)pyran-3,4-dione (4), diethoxy-2-methyl-propane (5), 2,3,4,5-tetrahydroxy-tetrahydro-pyran (6), and 3,4-dimethyl-2(3H)-furanone (7) were significantly increased by 170.18%, 58.21%, 7.74%, 139.50%, 23.16%, and 45.63%, respectively in the treated sample compared with the control sample. On the contrary, the peak area% of 2-hydroxy-γ-butyrolactone (3) was decreased by - 14.96% in the treated ashwagandha compared with the control sample. From the results, it can be hypothesized that The Trivedi Effect® - Biofield Energy Treatment might have the impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract and responsible for the alteration in the relative peak height/area of treated sample compared with the control sample. As a result, the concentrations of the phytoconstituents assumed to be increased in treated sample compared with the control sample. This treated ashwagandha root extract would be helpful for designing better nutraceutical/pharmaceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, immunological disorders, stress, arthritis, etc.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Magnesium gluconate is a potent antioxidant and widely used for the prevention and treatment of hypomagnesia. The current research was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on magnesium gluconate for the change in the physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably changed from - 24.96% to 99.98% compared with the control sample. The average crystallite size was significantly increased by 7.79% in the treated sample compared with the control sample. PSD analysis revealed that the particle sizes in The Trivedi Effect® Treated sample at d10, d50 and d90 values were decreased by 5.36%, 11.35% and 0.90%, respectively compared with the control sample. The surface area analysis revealed that surface area of the Biofield Energy Treated sample was significantly increased by 7.48% compared with the control sample. The FT-IR and UV-vis analysis showed that structure of the magnesium gluconate remained the same in both the treated and control samples. The TGA analysis revealed four steps thermal degradation of both the samples and the total weight loss of Biofield Energy Treated sample was increased by 0.12% compared with the control sample. The DSC analysis demonstrated that the melting temperature of the Biofield Energy Treated sample (171.29°C) was increased by 0.18% compared with the control sample (170.99°C). The latent heat of fusion was significantly increased by 27.09% in the treated sample compared with the control sample. The current study revealed that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) might lead to a new polymorphic form of magnesium gluconate, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. The Biofield Treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging, and other chronic infections.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Magnesium gluconate has the wide application for the prevention and treatment of hypomagnesemia. The objective of the current study was to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 minutes with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the percentage of the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly increased in treated sample by 80.38%, compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly increased compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.