Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Disodium hydrogen orthophosphate is a water soluble white powder widely used as pH regulator and saline laxative. The sodium nitrate is a highly water soluble white solid, used in high blood pressure, dentinal hypersensitivity, and production of fertilizers. The present study was aimed to investigate the impact of biofield treatment on spectral properties of disodium hydrogen orthophosphate and sodium nitrate. The study was performed in two groups i.e., control and treatment of each compound. The treatment groups were subjected to Mr. Trivedi’s biofield treatment. The spectral properties of control and treated groups of both compounds were studied using Fourier transform infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopic techniques. FT-IR spectrum of biofield treated disodium hydrogen orthophosphate showed the shifting in wavenumber of vibrational peaks (with respect to control) corresponding to O-H stretching from 2975 to 3357 cm-1, PO-H symmetrical stretching from 2359 to 2350 cm-1, O=P-OH deformation from 1717-1796 cm-1 to 1701-1735 cm-1, P=O asymmetric stretching from 1356 to 1260 cm-1 and P=O symmetric stretching from 1159 to 1132 cm-1, etc. Likewise, the FT-IR spectrum of sodium nitrate exhibited the shifting of vibrational frequency of N=O stretching from 1788 to 1648 cm-1 and NO3 asymmetric and symmetric stretchings from 1369 to 1381 cm-1 and 1340 to 1267 cm-1. UV spectrum of treated disodium hydrogen orthophosphate revealed a negative absorbance; it may be due to decrease in UV absorbance as compared to control. UV spectrum of control sodium nitrate exhibited two absorbance maxima (λmax) at 239.4 nm and 341.4 nm, which were altered to one absorbance maxima (λmax) at 209.2 nm after biofield treatment. Overall, the FT-IR and UV spectroscopic data of both compounds suggest an impact of biofield treatment on spectral properties with respect to force constant, bond strength, dipole moments and transition energy between two orbitals (ground state and excited state) as compared to respective control.

Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana

Klebsiella oxytoca (K. oxytoca) is a Gram-negative microbe generally associated with community and hospital-acquired infections. Due to its clinical significance, we evaluated the effect of biofield treatment on phenotype and biotype characteristics of K. oxytoca (ATCC 43165). The study was performed into three groups i.e. C (control), T1 (treatment, revived); and T2 (treatment, lyophilized). Subsequently, groups T1 and T2 were received biofield treatment and control group was remained as untreated. The antimicrobial sensitivity results showed 3.33% and 6.67% alteration in antimicrobials susceptibility in group T1 cells on day 5 and 10, respectively, and 3.33% alteration in antimicrobials susceptibility was observed in group T2 cells on day 10 as compared to control. The sensitivity patterns of cefazolin were changed from resistant (R) to intermediate (I) on day 5, and resistance (R) to susceptible (S) on day 10, in T1 cells of K. oxytoca. The MIC value of cefazolin was decreased by 2-fold in group T1 on day 10 as compared to control. The biofield treated K. oxytoca exhibited the changes in biochemical reactions about 3.03% and 15.15% of total tested biochemicals in group T1 cells on day 5 and 10, respectively as compared to control. The biotype number of K. oxytoca was altered in biofield treated group and organism identified as Raoultella ornithinolytica in T1 on day 10 as compared to control, which is the prominent finding of this study. These changes were found in treated bacteria that might be due to some alteration happened in metabolic/enzymatic pathway and/or at genetic level of K. oxytoca. Based on these data, it is speculated that biofiled treatment could be an alternative approach that can improve the effectiveness of the existing antimicrobials against the resistant pathogens.

Alice Branton, Dahryn Trivedi, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on four MDR clinical lab isolates (LS) of K. pneumoniae (LS 2, LS 6, LS 7, and LS 14). Samples were divided into two groups i.e. control and biofield treated. Control and treated groups were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed that there was 46.42% alteration in sensitivity of tested antimicrobials in treated group of MDR K. pneumonia isolates. MIC results showed an alteration in 30% of tested antimicrobials out of thirty after biofield treatment in clinical isolates of K. pneumoniae. An increase in antimicrobial sensitivity and decrease in MIC value was reported (in LS 6) in case of piperacillin/tazobactam and piperacillin. Biochemical study showed a 15.15% change in biochemical reactions as compared to control. A significant change in biotype numbers were reported in all four clinical isolates of MDR K. pneumoniae after biofield treatment as compared to control group. On the basis of changed biotype number after biofield treatment, new organism was identified as Enterobacter aerogenes in LS 2 and LS 14. These results suggest that biofield treatment has a significant effect on altering the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number of multidrug-resistant isolates of K. pneumoniae.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

m-toluic acid (MTA) is widely used in manufacturing of dyes, pharmaceuticals, polymer stabilizers, and insect repellents. The aim of present study was to evaluate the impact of biofield treatment on physical, thermal and spectroscopic properties of MTA. MTA sample was divided into two groups that served as treated and control. The treated group received Mr. Trivedi’s biofield treatment. Subsequently, the control and treated samples were evaluated using X-ray diffraction (XRD), surface area analyser, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) and ultraviolet-visible (UV-Vis) spectroscopy. XRD result showed a decrease in crystallite size in treated samples i.e. 42.86% in MTA along with the increase in peak intensity as compared to control. However, surface area analysis showed an increase in surface area of 107.14% in treated MTA sample as compared to control. Furthermore, DSC analysis results showed that the latent heat of fusion was considerably reduced by 40.32%, whereas, the melting temperature was increased (2.23%) in treated MTA sample as compared to control. The melting point of treated MTA was found to be 116.04°C as compared to control (113.51°C) sample. Moreover, TGA/DTG studies showed that the control sample lost 56.25% of its weight, whereas, in treated MTA, it was found 58.60%. Also, Tmax (temperature, at which sample lost maximum of its weight) was decreased by 1.97% in treated MTA sample as compared to control. It indicates that the vaporisation temperature of treated MTA sample might decrease as compared to control. The FT-IR and UV-Vis spectra did not show any significant change in spectral properties of treated MTA sample as compared to control. These findings suggest that biofield treatment has significantly altered the physical and thermal properties of m-toluic acid, which could make them more useful as a chemical intermediate.