Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Ragini Singh, Snehasis Jana

The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi’s biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis, surface area analysis, thermogravimetric analysis (TGA), elemental analysis, and Fourier transform infrared (FT-IR) spectroscopy. The XRD results showed 13.98% increase in crystallite size of treated sample (104.01 nm) as compared to the control (91.25 nm). The particle size data revealed an increase in d50 (average particle size) and d99 (size below which 99% particles are present) by 72.57% and 42.26%, respectively of the treated sample as compared to the control. Moreover, the surface area of the treated sample was reduced from 0.694 m2/g (control) to 0.560 m2/g in the treated sample. The TGA data showed the increase in onset temperature along with the reduction in the percent weight loss of the treated sample as compared to the control. Besides, the elemental analysis revealed the significant decrease in the percentage of nitrogen (10.47%) and hydrogen (9.35%) as well as the presence of sulphur in the treated sample. The FT-IR results showed the differences in the IR frequencies corresponding to pyridine ring and N-H2 deformation of the treated sample as compared to the control. Hence, the overall data revealed that the biofield energy treatment had a significant impact on the physicochemical properties of the treated sample that might help to improve its uses in the in vitro tissue culture techniques as compared to the control sample.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Ragini Singh, Snehasis Jana

2-chlorobenzonitrile (2-ClBN) is widely used in the manufacturing of azo dyes, pharmaceuticals, and as intermediate in various chemical reactions. The aim of present study was to evaluate the impact of biofield treatment on physical, thermal and spectroscopic properties of 2-ClBN. 2-ClBN sample was divided into two groups that served as treated and control. The treated group received Mr. Trivedi’s biofield treatment. Subsequently, the control and treated samples were evaluated using X-ray diffraction (XRD), surface area analyser, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) and ultraviolet-visible (UV-Vis) spectroscopy. XRD result showed a decrease in crystallite size in treated samples i.e. 4.88% in 2-ClBN along with the increase in peak intensity as compared to control. However, surface area analysis showed a decrease in surface area of 64.53% in treated 2-ClBN sample as compared to the control. Furthermore, DSC analysis results showed a significant increase in the latent heat of fusion (28.74%) and a slight increase in melting temperature (2.05%) in treated sample as compared to the control. Moreover, TGA/DTG studies showed that the control and treated 2-ClBN samples lost 61.05% and 46.15% of their weight, respectively. The FT-IR spectra did not show any significant change in treated 2-ClBN sample as compared to control. However, UV-Vis spectra showed an increase in the intensity of peak as compared to control sample. These findings suggest that biofield treatment has significantly altered the physical, thermal and spectroscopic properties of 2-ClBN, which could make them more useful as a chemical intermediate.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Bacillary dysentery and acute gastroenteritis caused by infection of Shigella species are major public health burden in India and its neighboring countries. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Shigella boydii (S. boydii) with respect of antimicrobial susceptibility assay, biochemical characteristics and biotyping. The American Type Culture Collection (ATCC 9207) strain of S. boydii was used in this experiment. The study was conducted in revived and lyophilized state of S. boydii. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. boydii were subjected to Mr. Trivedi’s biofield treatment. Gr. II was assessed on day 5 and day 10, while Gr. III on day 10 with respect to control (Gr. I). Sensitivity pattern of amoxicillin/k-clavulanate was improved from intermediate (I) to susceptible (S) with correspond to MIC value was also reduced by two folds (16/8 to ≤ 8/4 µg/mL) in both the treated groups as compared to control. The antimicrobial susceptibility of S. boydii showed 15% alteration in Gr. II on day 5, while significant (40%) alteration was found on day 10 as compared to control. The MIC values of antimicrobials for S. boydii also showed 12.50% alteration in Gr. II on day 5 while, significant alteration (59.38%) of minimum inhibitory concentration (MIC) value was found in Gr. II on day 10 as compared to control. It was observed that overall 69.70% biochemical reactions were changed in which 66.67% alteration was found in Gr. II on day 10 with respect to control. Moreover, biotype numbers were changed in all the treated groups without alteration of organism as compared to control. These results suggested that biofield treatment had significant impact on S. boydii in Gr. II on day 10 with respect to antimicrobial susceptibility, MIC and biochemical reactions pattern.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.