Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Ammonium acetate and ammonium chloride are the white crystalline solid inorganic compounds having wide application in synthesis and analytical chemistry. The aim of present study was to evaluate the impact of biofield treatment on spectral properties of inorganic salt like ammonium acetate and ammonium chloride. The study was performed in two groups of each compound i.e., control and treatment. Treatment groups were received Mr. Trivedi’s biofield treatment. Subsequently, control and treated groups were evaluated using Fourier Transform Infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopy. FT-IR spectrum of treated ammonium acetate showed the shifting in wavenumber of vibrational peaks with respect to control. Like, the N-H stretching was shifted from 3024-3586 cm-1 to 3033-3606 cm-1, C-H stretching from 2826-2893 cm-1 to 2817-2881 cm-1, C=O asymmetrical stretching from 1660-1702 cm-1 to 1680-1714 cm-1, N-H bending from 1533-1563 cm-1 to 1506-1556 cm-1 etc. Treated ammonium chloride showed the shifting in IR frequency of three distinct oscillation modes in NH4 ion i.e., at ν1, 3010 cm-1 to 3029 cm-1; ν2, 1724 cm-1 to 1741 cm-1; and ν3, 3156 cm-1 to 3124 cm-1. The N-Cl stretching was also shifted to downstream region i.e., from 710 cm-1 to 665 cm-1 in treated ammonium chloride. UV spectrum of treated ammonium acetate showed the absorbance maxima (λmax) at 258.0 nm that was shifted to 221.4 nm in treated sample. UV spectrum of control ammonium chloride exhibited two absorbance maxima (λmax) i.e., at 234.6 and 292.6 nm, which were shifted to 224.1 and 302.8 nm, respectively in treated sample. Overall, FT-IR and UV data of both compounds suggest an impact of biofield treatment on atomic level i.e., at force constant, bond strength, dipole moments and electron transition energy between two orbitals of treated compounds as compared to respective control.

Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

Indole compounds are important class of therapeutic molecules, which have excellent pharmaceutical applications. The objective of present research was to investigate the influence of biofield treatment on physical and thermal properties of indole. The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated samples were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy and ultraviolet-visible (UV-Vis) spectroscopy. XRD study demonstrated the increase in crystalline nature of treated indole as compared to control. Additionally, the treated indole showed increase in crystallite size by 2.53% as compared to control. DSC analysis of treated indole (54.45ºC) showed no significant change in melting temperature (Tm) in comparison with control sample (54.76ºC). A significant increase in latent heat of fusion (ΔH) by 30.86% was observed in treated indole with respect to control. Derivative thermogravimetry (DTG) of treated indole showed elevation in maximum thermal decomposition temperature (Tmax) 166.49ºC as compared to control (163.37ºC). This was due to increase in thermal stability of indole after biofield treatment. FT-IR analysis of treated indole showed increase in frequency of N-H stretching vibrational peak by 6 cm-1 as compared to control sample. UV spectroscopy analysis showed no alteration in absorption wavelength (λmax) of treated indole with respect to control. The present study showed that biofield has substantially affected the physical and thermal nature of indole.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Olga Mirgalijeva

The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a combination of the herbal root extract ashwagandha and minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the test formulation in splenocytes. The test formulation was divided into two parts; one was the control without the Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The MTT assay showed that the test formulation exhibited safe concentrations up to 1.053 µg/mL with cell viability ranging from 88.19% to 110.30% in the Biofield Energy Treated sample. The Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated test formulation. The expression of TNF-α was significantly inhibited by 7.23% at 1.053 µg/mL in the Biofield Energy Treated test formulation compared with the vehicle control. The level of TNF-α was significantly decreased by 3.90%, 11.74%, 3.12%, and 9.17% at 0.001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the untreated test formulation. Additionally, the expression of IL-1β was significantly reduced by 28.98%, 51.23%, 53.06%, 48.98%, 55.71%, and 59.10% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the vehicle control. Further, the Biofield Treated test formulation showed significant reduction of IL-1β by 35.07% (p≤0.05), 47.46% (p≤0.05), and 57.51% (p≤0.01) at 0.001053, 0.1053, and 1.053 µg/mL, respectively compared with the untreated test formulation. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppressive activity at 0.00001053, 0.0001053, 0.001053, and 0.01053 µg/mL by 19.38%, 24.97%, 31.23%, and 25.41%, respectively compared with the vehicle control group. The Biofield Treated test formulation significantly (p<0.001) reduced the MIP-1α expression by 19.33%, 22.57%, and 30.50% at 0.0001053, 0.001053, and 0.01053 µg/mL, respectively compared with the untreated test formulation. Overall, The Trivedi Effect®- Biofield Energy Healing (TEBEH)significantly down-regulated the pro-inflammatory cytokines and potentiated the immunosuppressive effect of the Biofield Energy Treated test formulation, which can be better utilized for organ transplants, autoimmune diseases, inflammatory disorders, anti-aging, stress management, overall health and quality of life, etc.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Sambhu Charan Mondal, Snehasis Jana

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.