Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

3-Nitroacetophenone (3-NAP) is an organic compound used as an intermediate for the synthesis of pharmaceutical agents. The aim of this study was to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of 3-NAP. The study was performed in two groups i.e. control and treated. The control group remained as untreated, and the treated group received Mr. Trivedi’s biofield energy treatment. The control and treated 3-NAP samples were further characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), laser particle size analyzer, surface area analyzer, Fourier transform infrared (FT-IR) spectroscopy, and ultra violet-visible spectroscopy (UV-vis) analysis. The XRD analysis showed decrease in crystallite size of treated 3-NAP by 20.27% as compared to the control sample. However, the XRD peaks of treated sample showed an increase in intensity as compared to the control. The DSC result showed a slight increase in melting temperature of treated 3-NAP (80.75ºC) with respect to the control (79.39ºC). The latent heat of fusion of treated 3-NAP was changed by 16.28% as compared to the control sample. The TGA analysis showed an increase in onset temperature of treated sample (192ºC) as compared to the control sample (182ºC). Further, the maximum thermal decomposition temperature (Tmax) of treated 3-NAP was increased as compared to the control. This showed the increase in thermal stability of treated 3-NAP with respect to control. The treated 3-NAP showed an increase in average particle size (d50) by 27.6% along with an increase in size exhibited by 99% of particles (d99) by 4.9% as compared to the control. Brunauer-Emmett-Teller (BET) analysis showed a substantial decrease in surface area by 24.6% with respect to the control. The FT-IR analysis showed an emergence of peak at 1558 cm-1 in treated 3-NAP sample as compared to the control. Nevertheless, the UV spectral analysis of treated 3-NAP showed no alterations in absorption peaks as compared to the control. Altogether, the result showed that biofield energy treatment has altered the physical, thermal and spectral properties of treated 3-NAP as compared to the control.

Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of 1,2,3-trimethoxybenzene. Methods: The study was performed by dividing the sample into two groups (control and treated). The control group remained as untreated, while the treated group received Mr Trivedi’s biofield energy treatment. The control and treated 1,2,3-trimethoxybenzene samples were then characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, and ultra violet-visible spectroscopy (UV-Vis) analysis. Results: XRD studies revealed the significant increase in crystallite size of treated sample by 45.96% as compared to the control sample. DSC analysis showed a decrease in melting temperature of the treated sample (45.93ºC) with respect to control (46.58ºC). Additionally, the substantial change was evidenced in latent heat of fusion of treated sample by 64.18% as compared to the control. TGA analysis indicated a decrease in maximum thermal decomposition temperature (Tmax) of treated sample (151.92ºC) as compared to the control sample (154.43ºC). This indicated the decrease in thermal stability of the treated sample as compared to the control. FT-IR spectroscopic analysis showed an increase in the frequency of C-O bond in treated sample (1105→1174 cm-1) as compared to the control sample. However, UV analysis showed no changes in absorption peaks in treated sample as compared to the untreated sample. Conclusion: Overall, the result indicated that biofield energy treatment has altered the physical, thermal and spectral properties of the treated sample as compared to control. Hence, the treated sample could be used as an intermediate in the synthesis of organic compounds.

Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

2, 6-Diaminopyridine (2, 6-DAP) has extensive use in synthesis of pharmaceutical compounds. The objective of present research was to investigate the influence of biofield treatment on physical, thermal and spectral properties of 2, 6-DAP. The study was performed in two groups, control and treated. The control group remained as untreated, and biofield treatment was given to treatment group. The control and treated 2, 6-DAP samples were characterized by X-ray diffraction (XRD), Differential scanning calorimetry (DSC), Thermo gravimetric analysis (TGA), Laser particle size analyzer, surface area analyzer, Fourier transform infrared (FT-IR) spectroscopy, and UV-visible spectroscopy. XRD analysis revealed decrease in intensity of the peaks of treated 2, 6-DAP with respect to control. Unit cell volume and molecular weight were decreased by 2.97% and 2.98% respectively in treated 2, 6-DAP as compared to control. Crystallite size was decreased by 24.70% in treated 2, 6-DAP with respect to control. DSC analysis showed no significant change in melting temperature of treated 2, 6-DAP with respect to control. Nevertheless, the treated 2, 6-DAP showed significant increase in latent heat of fusion by 35.52% as compared to control 2, 6-DAP. TGA analysis showed decrease in percent weight loss of the treated 2, 6-DAP in comparison with control. Additionally, substantial increase in maximum thermal decomposition temperature (Tmax) was observed in treated 2, 6-DAP (203.52°C) as compared with control 2, 6-DAP (186.84°C). Particle size analysis results showed a substantial decrease in d50 (average particle size) and d99 (size exhibited by 99% of the particles) of the treated 2, 6-DAP by 20.5 and 57.4%, respectively as compared to control. Additionally, the BET analysis showed substantial increase in surface area of treated 2, 6-DAP by 75.58% as compared to control. FT-IR spectrum of treated 2, 6-DAP showed alteration in O-H stretching (3390→3370 cm-1), C-H stretching (3132→3138 cm-1) and N-H bending (1637→1604 cm-1) vibration peaks with respect to control. However, UV-visible analysis of treated 2, 6-DAP showed no significant changes in absorption peaks (λ max) with respect to control. Overall, the results demonstrated that biofield has significant impact on the physical, thermal and spectral properties of the treated 2, 6-DAP.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Study background: Multidrug resistant Escherichia coli (MDR E. coli) has become a major health concern, and failure of treatment leads to huge health burden. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on E. coli. Methods: Four MDR clinical lab isolates (LSs) of E. coli (LS 8, LS 9, LS 10, and LS 11) were taken and divided into two groups i.e. control and biofield treated. Control and treated samples were identified with respect to its antimicrobial sensitivity assay, biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with its respective control group. Results: Antimicrobial sensitivity assay showed 50% alteration in sensitivity of total tested antimicrobials in treated group of MDR E. coli isolates. MIC results showed the alteration in MIC of about 40.63% antimicrobials out of thirty two tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Ticarcillin/k-clavulanate showed improved sensitivity (R → I) with decreased MIC value in LS 9 as compared to control. A fourfold and twofold decreased in MIC values were reported in case of piperacillin/tazobactam (in LS 9) and chloramphenicol (in LS 8), respectively as compared to respective control. Biochemical study showed a 39.39% alteration in biochemical reactions after treatment among four isolates of E. coli as compared to control. A significant change in biotype numbers were reported in three clinical isolates (i.e. LS 8, LS 9, and LS 11) of MDR E. coli as compared to control. On the basis of changed biotype number (7774 5272) after biofield treatment, organism with maximum probability was identified as Enterobacter aerogenes in LS 8 as compared to control, (E. coli, 7711 5012). Conclusion: Overall results suggest that Mr Trivedi’s biofield treatment has a significant effect on altering the antimicrobial sensitivity, biochemical reactions and biotype number of MDR isolates of E. Coli.