Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the samples (control and treated) were evaluated using various analytical techniques such as X-ray diffractometry (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis- differential thermal analysis (TGA-DTA), UV-Vis spectrometry, and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis showed the crystalline nature of both control and treated samples of PMM. The X-ray diffractogram showed the significant increase in the intensity of XRD peaks in treated sample as compared to the control. The XRD analysis revealed 6.64% increase in the average crystallite size of treated PMM with respect to the control. The DSC analysis showed about 8.66% decrease in the latent heat of fusion in treated sample with respect to the control. The TGA-DTA analysis exhibited about 4.71% increase in onset temperature of thermal degradation after biofield treatment with respect to the control, while the maximum thermal degradation temperature (Tmax) was also increased (5.06%) in treated sample with respect to the control. This increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. The UV spectroscopic study showed the slight blue shift in λmax of treated sample with respect to the control. FT-IR spectrum of control PMM showed the peak at 3132 cm-1 (C-H stretching) that was observed at higher wavenumber i.e. at 3161 cm-1 in the treated sample. Other vibrational peaks in the treated sample were observed in the similar region as that of the control. Altogether, the XRD, DSC, TGA-DTA, UV-Vis, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physicochemical properties of PMM. This treated PMM might be more effective as a micropropagation medium as compared to the control.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentioned parameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. The antimicrobial sensitivity assay was reported with 46.67% alteration (14 out of 30 tested antimicrobials) in treated group of MDR E. coli isolates. The minimum inhibitory concentration (MIC) study showed the alteration in MIC values of about 34.37% (11 out of 32) tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Piperacillin/tazobactam was reported with improved sensitivity and four-fold decrease in the MIC value (64 to ≤16 µg/mL) in LS 42, as compared with the control. Amoxicillin/k-clavulanate reported with improved sensitivity pattern from resistance to susceptible, with two-fold decrease in MIC value (>16/8 to ≤8/4 µg/mL) in biofield treated LS 51. Further, biochemical study showed 24.24% alteration (8 out of 33) in tested biochemical reactions after treatment among four isolates of E. coli as compared to the control. A change in biotype number (7774 4272) was reported as compared to the control, (7311 4012), with new organism identified as Klebsiella pneumoniae in biofield treated LS 13 with respect to the control organism, E. coli. Overall, data suggested that Mr. Trivedi’s biofield energy treatment can be applied to alter the antimicrobial sensitivity, biochemical reactions and biotype number of E. Coli.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Gunin Saikia, Snehasis Jana

N,N-Dimethylformamide (DMF) is a ‘universal’ solvent and has wide variety of applications in organic synthesis, purification, crystallization, and as cross-linking agent. The aim of this study was to evaluate the physicochemical and spectroscopic properties of DMF after the biofield energy treatment using various analytical techniques. DMF sample was divided into two parts, one part (control) remained as untreated, while the other (treated) part was treated with Mr. Trivedi’s biofield energy treatment. The treated sample was subdivided into three parts named as T1, T2, and T3 for gas chromatography-mass spectrometry (GC-MS) analysis. Five relatively intense peaks were observed in the mass spectrum of both control and treated samples of DMF. The GC-MS data revealed that the isotopic abundance ratio of (PM+1)/PM in DMF was slightly decreased by 5.76% in T1, and increased by 48.73%, and 30.17% in T2, and T3 samples, respectively as compared to the control [where, PM- primary molecule, (PM+1)- isotopic molecule either for 13C or 2H or 15N]. Similarly, the isotopic abundance ratio of (PM+2)/PM was decreased by 10.34% in T1 and then increased upto 43.67% (T2) as compared to the control [where, (PM+2)- isotopic molecule for 18O]. In high performance liquid chromatography (HPLC), the treated DMF showed similar retention time (TR) as compared to the control with an additional small peak at 2.26 min appeared in the treated sample. In DSC thermogram the heat change in a sharp endothermic transition at around 61°C of treated DMF was increased by 152.56% as compared to the control. Further, C=O and C-N stretching frequencies of treated sample were shifted by 7 cm-1 and 3 cm-1, respectively towards low energy region in Fourier transform infrared (FT-IR) spectroscopy. These results suggested that biofield energy treatment has significantly altered the physical and spectroscopic properties of DMF, which could make them more stable solvent in organic synthesis and as a suitable formulation agent in polymer/paint industry.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Viral load quantification is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of AIDS. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield treatment on HIV-1 and HCMV in terms of viral loads as surrogate marker. The viral load assay was performed on stored stock cultures of HIV infected human plasma samples before and after 7 days of biofield treatment using Roche COBAS® AMPLICOR analyzer. Viral load (HIV-1 RNA and HCMV DNAaemia) was considered as surrogate marker for assessment of the impact of Mr. Trivedi’s biofield treatment in HIV infected stored plasma samples. The viral load quantification of HIV-1 RNA in infected stored plasma samples was significantly reduced by 65% in biofield treated group as compared to control. Additionally, viral load of HCMV DNAaemia in infected stored plasma samples was also reduced by 80% in the biofield treated group as compared to control. Because, children are more prone to HCMV infection and adults are generally liable to suffer from HIV-1 infection. As the biofield treatment has reduced HCMV DNAaemia, it could be beneficial for HIV infected children populations. Altogether, data suggest that biofield treatment has significantly reduced the viral load quantification in HIV-1 and HCMV infected stored plasma samples and could be a suitable alternative treatment strategy for AIDS patients in near future.