Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Magnesium gluconate is a pharmaceutical/nutraceutical compound used as a source of magnesium ion. The recent study described the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment on magnesium gluconate for the variation in physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with The Trivedi Effect®-Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably altered from -70% to 130% compared with the control sample. The average crystallite size was significantly decreased by 23.74% in the treated sample compared to the control sample. Biofield Energy Healing Treatment significantly reduced the particle size of magnesium gluconate at d10, d50, and d90 values by 12.15%, 8.98% and 15.35%, respectively compared to the control sample. The surface area analysis showed that surface area of the treated sample was significantly increased by 11.76% compared with the control sample. The FT-IR and UV-vis analysis displayed that structure of the magnesium gluconate persisted identical in both the treated and control samples. The TGA analysis exhibited four steps thermal degradation in both samples and the total weight loss of the Biofield Energy Treated sample was reduced by 0.19% compared with the control sample. The melting temperature of the Biofield Energy Treated sample (171.25ºC) was slightly (0.16%) higher from the control sample (170.97ºC). The latent heat of fusion was significantly decreased by 7.76% in the treated sample compared to the control sample. The TGA and DSC analysis revealed that the thermal stability of the treated sample was enhanced compared with the control sample. The current study revealed that The Trivedi Effect®-Energy of Consciousness Healing Treatment might produce a new polymorphic form of magnesium gluconate, which could be more soluble and bioavailable along with improved thermal stability compared with the untreated compound. The Biofield Treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging, and other chronic infections.

Dennille Mellesia Smith, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

The current study was attempted to evaluate the impact of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based herbomineral test formulation and cell medium (DMEM) against skin health. The test formulation and DMEM were divided into two parts. One of each part was received the Consciousness Energy Healing Treatment by Dennille Mellesia Smith and was termed as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test items. MTT showed >78% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was significantly increased by 238.30%, 192.06%, and 43.96% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of collagen was significantly increased by 55.55%, 32.65%, and 52.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the untreated group. Elastin was significantly (p≤0.001) increased by 6.30%, 105.04%, and 29.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was increased by 4.78%, 29.71%, and 58.29% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test Formulation group. The level of melanin was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively at 0.13 µg/mL compared to the untreated group. Skin protection against UV-B data displayed that cell proliferation was increased by 17.88%, 20.14%, and 9.89% in the BT-DMEM + BT-Test formulation at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Wound healing activity exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, result suggests that the Biofield Energy Treated DMEM and test formulation exhibited better responses compared to the untreated medium and test formulation. Therefore, the Biofield Energy Treated herbomineral formulation could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.

Dezi Ann Koster, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

The application of the herbomineral formulations in general skin health are increasing day-by-day due to the excellent outcomes without any adverse effects. This study was designed to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral formulation and cell medium against various skin health parameters. The test formulation consists of minerals (zinc chloride, sodium selenate, and sodium molybdate) and L-ascorbic acid along with herbal extracts, Centella asiatica, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, one was treated with a Biofield Treatment (BT) by Dezi Ann Koster and denoted as treated, while the other part was coded as the untreated (UT) groups. MTT assay results showed that test formulation was safe and nontoxic with more than 89% cell viability in the tested cell lines. BrdU assay showed an improved cell proliferation by 2.82% in the UT-DMEM + BT-Test formulation group compared with the untreated group. The level of collagen was significantly increased by 32.42%, 33.64%, and 29.13% at 2.5, 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation, while34.17%, 26.73%, and 17.56% increased at 2.5, 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Elastin level was increased by 408.6% at concentration of 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with untreated group. However, hyaluronic acid (HA) level was increased by 31.88%, 15.52%, and 58.29% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared with untreated group. Besides, melanin synthesis was significantly inhibited by 16.09% and 18.93% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.125 µg/mL compared with the untreated group. Anti-wrinkling activity in HFF-1 cells showed an improved cell viability by 5.49% and 11.26% at 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Wound healing scratch assay results showed a significant healing rate by 5% and 10% in HFF-1 and HaCaT cells lines, respectively with high cellular migration of fibroblast and keratinocytes. Overall, it can be concluded that the Biofield Energy Healing (The Trivedi Effect®) based test formulation and cell medium could be helpful against various skin disorders and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent.