Shrikant Patil, Harish Shettigar, Sambhu Charan Mondal, Snehasis Jana

Study background: Nowadays, hepatitis is a major challenge for clinical research, regulatory bodies, and clinicians who are trying to assess the more effectiveness of antiviral therapy against patients. Viral load count is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of hepatitis. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield modality on hepatitis B virus (HBV) and hepatitis C virus (HCV) in terms of viral load as surrogate marker. Method: The viral load assay was performed on stock human plasma samples of HBV and HCV before and after 7 days of biofield treatment using Roche COBAS® AMPLICOR analyzer according to manufacturer’s instructions. Viremia (viral DNA for HBV, RNA for HCV) was considered as surrogate marker for assessment of the impact of Mr. Trivedi’s biofield treatment. Result: The viral load of HBV DNA in infected plasma samples showed a significant alteration in the biofield treated group as compared to control. Additionally, viral load count of HCV RNA in infected plasma samples was significantly reduced by 67% in the biofield treated group as compared to control. As the biofield treatment has significantly reduced HCV RNA, it could be beneficial for particularly HCV infected populations. Conclusion: Altogether, data suggest that biofield treatment has significantly alteration in HBV and reduced the viral load count in HCV infected plasma samples and could be a suitable alternative treatment strategy for hepatitis patients in near future.

Shrikant Patil

Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain and were analyzed within 10 days after incubation. Results: The results indicated that Mr.Trivedi's energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through an automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi's energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Treatment groups received the biofield treatment, and control group was remained as untreated. Mini-API ID32C strip employed for evaluation of antifungal sensitivity and minimum inhibitory concentration (MIC). The results showed that sensitivity of Candida albicans in T1 cells was changed against itraconazole from intermediate (I) to resistance (R) on day 10. The Candida kefyr exhibited a change in susceptibility against itraconazole in T2 cell from S→I, on day 10. Likewise, Candida krusei showed the alterations in sensitivity against two antifungal drugs: fluconazole from S→I (T1 on day 10) and itraconazole S→I (T1 and T2 on all assessment days). The Cryptococcus neoformans changed from S→I in T1 cell on day 5 and 10, against itraconazole. Sensitivity of Candida tropicalis was also altered from I→R against flucytosine (T1 and T2, on all assessment days). Similarly, Saccharomyces cerevisae altered from S→I (T1) and S→R (T2) on day 10. The MIC values of antifungal drugs were altered in the range of 2-8 folds, as compared to the control. Fungal identification data showed the significant changes in species similarity of few tested fungi as C. albicans changed from 91.9% to 98.5 and 99.9% in T1 and T2 cells, respectively on day 10. C. krusei was changed from 97.9% to 85.9% (T2 day 10), and C. tropicalis was altered from 88.7% to 99.6% (T1 day 5) and 99.0% (T2). These findings suggest that biofield treatment could be applied to alter the susceptibility pattern of antifungal drug therapy in future.

Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

The methylsulfonylmethane (MSM) is an organosulfur compound having sulfonyl functional group. It is occurred naturally in some primitive plants and used in disease related to chronic pain, inflammation, and arthritis. This study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MSM. The study was performed in two groups viz. the control group was remained as untreated, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. After that, both the control and treated samples were analyzed using surface area analyzer, X-ray diffraction (XRD), thermogravimetric analysis-derivative thermogravimetry (TGA-DTG), differential scanning calorimetry (DSC), and Fourier transform infrared (FT-IR) spectroscopy. The surface area analysis exhibited a significant decrease in the surface area of treated sample by 22.96% as compared to the control. The XRD analysis showed the significant increase in average crystallite size by 49.20% in the treated sample with respect to the control. The DSC analysis showed the significant increase (67.20%) in latent heat of fusion of treated sample with respect to the control. The TGA analysis showed the onset temperature of thermal degradation at 170°C in the control sample that was slightly decreased to 168.05°C after biofield treatment. Moreover, the Tmax (maximum thermal degradation temperature) was also decreased slightly from 186.66°C (control) to 183.38°C (treated). This indicated the early phase of vaporization in treated sample as compared to the control. The FT-IR spectroscopic study exhibited the alteration in wavenumber of S=O group that suggests the effect of biofield treatment on force constant and bond strength of MSM molecules. Altogether, the surface area, XRD, thermal analysis and FT-IR spectroscopy suggests that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal, and spectral properties of MSM.