Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Ragini Singh, Snehasis Jana

The hydrolysed vegetable proteins are acidic or enzymatic hydrolytic product of proteins derived from various sources such as milk, meat or vegetables. The current study was designed to evaluate the impact of biofield energy treatment on the various physicochemical and spectra properties of Hi VegTM acid hydrolysate i.e. a hydrolysed vegetable protein. The Hi VegTM acid hydrolysate sample was divided into two parts that served as control and treated sample. The treated sample was subjected to the biofield energy treatment and its properties were analysed using particle size analyser, X-ray diffraction (XRD), surface area analyser, UV-visible and infrared (FT-IR) spectroscopy, and thermogravimetric analysis. The results of various parameters were compared with the control (untreated) part. The XRD data showed the decrease in crystallite size of treated sample from 110.27 nm (control) to 79.26 nm. The particle size was also reduced in treated sample as 162.13 μm as compared to the control sample (168.27 μm). Moreover, the surface area analysis revealed the 63.79% increase in the surface area of the biofield treated sample as compared to the control. The UV-Vis spectra of both samples i.e. control and treated showed the absorbance at same wavelength. However, the FT-IR spectroscopy revealed the shifting in peaks corresponding to N-H, C-H, C=O, C-N, and C-S functional groups in the treated sample with respect to the control. The thermal analysis also revealed the alteration in degradation pattern along with increase in onset temperature of degradation and maximum degradation temperature in the treated sample as compared to the control. The overall data showed the impact of biofield energy treatment on the physicochemical and spectroscopic properties of the treated sample of Hi VegTM acid hydrolysate. The biofield treated sample might show the improved solubility, wettability and thermal stability profile as compared to the control sample.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Yeast extract powder (YE powder) is particularly used in culture media for the cultivation of microorganisms found in milk or other dairy products. The present study was intended to explore the influence of biofield energy treatment on the physicochemical and spectral properties of YE powder. The study was accomplished in two groups; first group was remained as control, while another was subjected to Mr. Trivedi’s biofield energy treatment and termed as the treated group. Afterward, both the samples were evaluated using several analytical techniques. The X-ray diffractometry (XRD) study showed the halo patterns of XRD peaks in both the samples. This indicated the amorphous nature of the samples. The particle size study revealed the 4.77% and 26.28% increase d50 (in the average particle size) and d99 (particle size below that 99% particles are present), respectively of treated YE powder with respect to the control. The surface area analysis showed the 14.06% increase in the specific surface area of treated sample with respect to the control. The differential scanning calorimetry (DSC) analysis exhibited the 41.64% increase in the melting temperature of treated YE powder sample as compared to the control. The TGA/DTG analysis exhibited the increase in Tonset (onset temperature of thermal degradation) by 7.51% and 12.45% in first and second step of thermal degradation, respectively in the treated sample as compared to the control. Furthermore, the Tmax (maximum thermal degradation temperature) was increased by 4.16% and 24.79% in first and second step of thermal degradation, respectively in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as C-H (stretching) from 2895→2883 cm-1 and 2815→2831 cm-1, respectively; C-N from 1230→1242 cm-1; and C-O stretching from 1062-1147 cm-1→1072-1149 cm-1 of treated YE powder sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (λmax) in both the control and treated samples. Therefore, the analytical results suggested the considerable impact of Mr. Trivedi’s biofield energy treatment on physicochemical and spectral properties of YE powder. The increase in Tonset and Tmax after the biofield treatment suggests that the treated YE powder might be more effective in culture medium than the control YE powder.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

Phosphate buffer saline (PBS) has numerous biological and pharmaceutical applications. Hank buffer salt (HBS) has been used as a medium for tissue culture applications. This research study was aimed to investigate the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of the PBS and HBS. The study was executed in two group’s i.e. control and treated. The control group was kept aside as control and treated group had received the biofield energy treatment. The control and treated samples were further characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis indicated the increase in crystallite size by 5.20% in treated PBS as compared to the control. Similarly, the treated HBS also showed increase in crystallite size by 3.20% with respect to the control. Additionally, the treated PBS showed an increase in Bragg’s angle (2θ) as compared to the control sample. However, a decrease in Bragg’s angle of XRD peaks of the treated sample was noticed in the treated HBS. The DSC analysis of the control PBS showed melting temperature at 224.84°C; however melting temperature was not observed in the treated sample. However, DSC analysis of the treated HBS showed an increase in melting temperature (152.83°C) in comparison with the control (150.60°C). Additionally, the latent heat of fusion of the treated HBS was increased substantially by 108.83% as compared to the control. The TGA thermogram of the treated PBS showed an increase in onset of thermal degradation (212°C) as compared to the control (199°C). Whereas, the treated HBS showed less weight loss comparing with the control sample. This indicated the increase in thermal stability of the both the treated PBS and HBS samples. The FT-IR spectroscopic analysis of treated PBS showed alterations in the frequency of the functional groups such as O-H, C-H, P=O, O=P-OH, and P-OH as compared to the control. Additionally, the FT-IR spectrum of the treated HBS showed increase in frequency of calcium chloride phase (1444→1448 cm-1) as compared to the control sample. Altogether, it was observed that biofield energy treatment had caused physical, thermal and spectral changes in the treated samples as compared to the control. It is assumed that biofield energy treated PBS and HBS could be a good prospect for biological and tissue culture applications.

Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Gunin Saikia, Snehasis Jana

O-Toluic acid (OTA) and m-toluic acid (MTA) are two isomers of toluic acid that act as an important organic intermediates, mostly used in medicines and pesticides. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, (PM+1)/PM and 18O/16O, (PM+2)/PM, in toluic acid isomers using gas chromatography-mass spectrometry (GC-MS). The OTA and MTA samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to biofield energy treatment. The GC-MS spectra of both the isomers showed five m/z peaks due to the molecular ion peak and fragmented peaks of toluic acid derivatives. The isotopic abundance ratio of (PM+1)/PM and (PM+2)/PM were calculated for both the isomers and found significant alteration in the treated isomers. The isotopic abundance ratio of (PM+1)/PM in treated samples of OTA was decreased and then slightly increased upto 2.37% in T2, where the (PM+2)/PM in treated OTA, significantly decreased by 55.3% in T3 sample. Similarly, in case of MTA, the isotopic abundance ratio of (PM+1)/PM in the treated sample showed a slight increase the (PM+2)/PM was decreased by 11.95% in T2 as compared to their respective control. GC-MS data suggests that the biofield energy treatment on toluic acid isomers had significantly altered the isotopic abundance of 2H, 13C, and 18O in OTA and MTA as compared to the control.