Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Magnesium gluconate is a potent antioxidant and widely used for the prevention and treatment of hypomagnesia. The current research was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on magnesium gluconate for the change in the physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably changed from - 24.96% to 99.98% compared with the control sample. The average crystallite size was significantly increased by 7.79% in the treated sample compared with the control sample. PSD analysis revealed that the particle sizes in The Trivedi Effect® Treated sample at d10, d50 and d90 values were decreased by 5.36%, 11.35% and 0.90%, respectively compared with the control sample. The surface area analysis revealed that surface area of the Biofield Energy Treated sample was significantly increased by 7.48% compared with the control sample. The FT-IR and UV-vis analysis showed that structure of the magnesium gluconate remained the same in both the treated and control samples. The TGA analysis revealed four steps thermal degradation of both the samples and the total weight loss of Biofield Energy Treated sample was increased by 0.12% compared with the control sample. The DSC analysis demonstrated that the melting temperature of the Biofield Energy Treated sample (171.29°C) was increased by 0.18% compared with the control sample (170.99°C). The latent heat of fusion was significantly increased by 27.09% in the treated sample compared with the control sample. The current study revealed that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) might lead to a new polymorphic form of magnesium gluconate, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. The Biofield Treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging, and other chronic infections.

Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Magnesium gluconate has the wide application for the prevention and treatment of hypomagnesemia. The objective of the current study was to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 minutes with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the percentage of the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly increased in treated sample by 80.38%, compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly increased compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.

Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Withania somnifera (Ashwagandha) root extract contains lot of biologically active metabolites, which have a broad range of pharmacological activities. The current study was designed to evaluate the effects of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that both the treated and control samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were decreased by 8.41%, 0.51%, and 7.88%, respectively compared with the control sample. The surface area analysis revealed that the surface area of the treated sample was significantly increased by 5% compared to the control sample. The FT-IR analysis indicated the alteration of the force constant for the functional groups of the treated sample in comparison to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.3 and 205.0 nm, respectively. The TGA analysis revealed that the total weight loss was decreased by 0.65% in the treated sample compared with the control sample. The DSC analysis indicated that the onset, peak, and endset vaporization temperature of the treated sample were significantly increased by 41.97%, 23.51%, and 8.82%, respectively as compared to the control sample. The latent heat of vaporization (∆H) of the treated (239.96 J/g) sample was significantly increased by 39.84% compared with the control (171.60 J/g) sample. This indicated that the treated sample was thermally more stable as compared to the control sample. The current findings suggested that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) might have the astounding capacity to enhance the solubility, absorption, dissolution, and bioavailability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Sodium selenate is an important nutraceutical/pharmaceutical compound used for the prevention and treatment of cancer, diabetes, inflammatory diseases, etc. The objective of the current study was to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing treatment) on physical, structural, thermal, and behavioral properties of sodium selenate using PXRD, PSD, FT-IR, UV-vis, TGA, and DSC analysis. Sodium selenate was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis showed a significant alteration of the crystallite size of the treated sample in the range of -34.41% to 33.27% compared to the control sample. However, the average crystallite size of the treated sample was significantly decreased by 7.85% compared with the control sample. The particle size of the treated sample at d10, d50, and d90 values were significantly reduced by 4.72%, 8.40%, and 32.33%, respectively compared with the control sample. Consequently, the surface area of the treated sample was significantly increased by 6.25% compared to the control sample. The control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 886 cm-1 and 887 cm-1, respectively due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of the control and treated samples were at 204.6 and 204.9 nm, respectively. TGA analysis revealed that the total weight loss of the treated sample was reduced significantly by 5.64% compared with the control sample. The DSC analysis showed that the treated sample (94.63°C) had very close vaporization temperature than the control sample (94.97°C). But, the latent heat of vaporization was increased significantly in the treated sample by 7.06% compared to the control sample. Thus, The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to generate a new polymorphic form of sodium selenate, which would be more soluble, bioavailable, and thermally stable compared with the untreated sample. The Trivedi Effect® treated sodium selenate would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, stress, aging, infectious diseases, cancer, diabetes, heart diseases, Alzheimer’s disease, etc.