Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Omprakash Latiyal, Snehasis Jana

Ethanol is a polar organic solvent, and frequently used as a fuel in automobile industries, principally as an additive with gasoline due to its higher octane rating. It is generally produced from biomass such as corn, sugar and some other agriculture products. In the present study, impact of biofield treatment on ethanol was evaluated with respect to its atomic and thermal properties. The ethanol sample was divided into two parts i.e., control and treatment. Control part was remained untreated. Treatment part was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using Gas chromatography-mass Spectrometry (GC-MS), Differential scanning calorimetry (DSC), and High performance liquid chromatography (HPLC). GC-MS data revealed that isotopic abundance of 13C i.e., δ13C of treated ethanol was significantly changed from -199‰ upto 155‰ as compared to control. The DSC data exhibited that the latent heat of vaporization of treated ethanol was increased by 94.24% as compared to control, while no significant change was found in boiling point. Besides, HPLC data showed that retention time was 2.65 minutes in control, was increased to 2.76 minutes in treated ethanol sample. Thus, overall data suggest that biofield treatment has altered the atomic and thermal properties of ethanol.

Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Harish Shettigar, Mayank Gangwar, Snehasis Jana

In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Five MDR clinical lab isolates (LS 22, LS 23, LS 38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated. Control and treated group were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed 60% alteration in sensitivity of tested antimicrobials in MDR isolates of P. aeruginosa after biofield treatment. MIC results showed an alteration in 42.85% tested antimicrobials out of twenty eight after biofield treatment in five isolates of MDR P. aeruginosa. Biochemical study showed a 48.48% change in tested biochemical reactions out of thirty three as compared to control. A significant change in biotype numbers was reported in three clinical lab isolates of MDR P. aeruginosa out of five, after biofield treatment as compared to respective control. On the basis of changed biotype number (7302 0052) in biofield treated LS 23, new organism was identified as Citrobacter freundii as compared to control (0206 3336). A very rare biotype number (7400 4263) was found in biofield treated LS 38, as compared to control (0206 3736). Study results suggest that biofield treatment on lab isolates of MDR P. aeruginosa has significant effect on the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number. Biofield treatment might prevent the emergence of absolute resistance pattern of useful antimicrobials against MDR isolates of P. aeruginosa.

Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

3-Chloro-4-fluoroaniline (CFA) is used as an intermediate for the synthesis of pharmaceutical compounds. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of CFA. The study was performed in two groups (control and treated). The control group remained as untreated, and the treated group received Mr. Trivedi’s biofield energy treatment. The control and treated CFA samples were further characterized by x-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), fourier transform infrared (FT-IR) spectroscopy, and ultra violet-visible spectroscopy (UV-vis) analysis. The XRD analysis of treated CFA showed significant changes in the intensity of peaks as compared to the control. However, the average crystallite size (G) was significantly decreased by 22.08% in the treated CFA with respect to the control. The DSC analysis showed slight decrease in the melting temperature of treated CFA (47.56°C) as compared to the control (48.05°C). However, the latent heat of fusion in the treated sample was considerably changed by 4.28% with respect to the control. TGA analysis showed increase in maximum thermal decomposition temperature (Tmax) of the treated sample (163.34°C) as compared to the control sample (159.97°C). Moreover the onset temperature of treated CFA (148 °C) was also increased as compared to the control sample (140°C). Additionally, the weight loss of the treated sample was reduced (42.22%) with respect to the control (56.04%) that may be associated with increase in thermal stability. The FT-IR spectroscopic evaluation showed emergence of one new peak at 3639 cm-1 and alteration of the N-H (stretching and bending) peak in the treated sample as compared to the control. Overall, the result demonstrated that Mr. Trivedi’s biofield energy treatment has paramount influence on the physical, thermal and spectral properties of CFA.

Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Gunin Saikia, Snehasis Jana

The aim of the present study was to evaluate the impact of biofield energy treatment on the thermal, spectroscopic, and chemical properties of benzophenone. The study was done using various analytical methods such as gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-Vis) spectroscopy. The benzophenone sample was divided into two parts, one part was subjected to Mr. Trivedi’s biofield energy treatment, called as treated and the other part was remained as untreated, called as control. Mass spectra showed the molecular ion peak at m/z = 182 in control and all the treated benzophenone samples with different intensities (treated samples further divided in to three parts, T1, T2, and T3 for GC-MS study). The isotopic abundance ratio of 2H/1H, 13C/12C (PM+1)/PM and in treated sample was decreased by 44.87% in T2 and slightly increased upto 5.79% in case of T1 as compared to the control [where, PM- primary molecule, (PM+1)- isotopic molecule either for 13C or 2H]. Moreover, isotopic abundance ratio of 18O/16O (PM+2)/PM in the treated sample was increased up to 22.64% in T3. The retention time of treated benzophenone was slightly increased (0.88 min) as compared to the control in HPLC chromatogram. The DSC data exhibited that the heat of degradation of treated benzophenone was increased by 674.16% as compared to the control. While, C=O stretching frequency of treated sample was shifted by 6 cm-1 to low energy region in FT-IR spectroscopy. Further, the UV-Vis spectra of control sample showed characteristic absorption peaks at 210 nm and 257 nm that was blue shifted to 205 nm and 252 nm, respectively in the treated sample. These results suggested that biofield treatment has significantly altered the thermal, spectroscopic, and chemical properties of benzophenone, which could make them more useful as reaction intermediate in industrial applications.