Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentioned parameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. The antimicrobial sensitivity assay was reported with 46.67% alteration (14 out of 30 tested antimicrobials) in treated group of MDR E. coli isolates. The minimum inhibitory concentration (MIC) study showed the alteration in MIC values of about 34.37% (11 out of 32) tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Piperacillin/tazobactam was reported with improved sensitivity and four-fold decrease in the MIC value (64 to ≤16 µg/mL) in LS 42, as compared with the control. Amoxicillin/k-clavulanate reported with improved sensitivity pattern from resistance to susceptible, with two-fold decrease in MIC value (>16/8 to ≤8/4 µg/mL) in biofield treated LS 51. Further, biochemical study showed 24.24% alteration (8 out of 33) in tested biochemical reactions after treatment among four isolates of E. coli as compared to the control. A change in biotype number (7774 4272) was reported as compared to the control, (7311 4012), with new organism identified as Klebsiella pneumoniae in biofield treated LS 13 with respect to the control organism, E. coli. Overall, data suggested that Mr. Trivedi’s biofield energy treatment can be applied to alter the antimicrobial sensitivity, biochemical reactions and biotype number of E. Coli.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gunin Saikia, Snehasis Jana

N,N-Dimethylformamide (DMF) is a ‘universal’ solvent and has wide variety of applications in organic synthesis, purification, crystallization, and as cross-linking agent. The aim of this study was to evaluate the physicochemical and spectroscopic properties of DMF after the biofield energy treatment using various analytical techniques. DMF sample was divided into two parts, one part (control) remained as untreated, while the other (treated) part was treated with Mr. Trivedi’s biofield energy treatment. The treated sample was subdivided into three parts named as T1, T2, and T3 for gas chromatography-mass spectrometry (GC-MS) analysis. Five relatively intense peaks were observed in the mass spectrum of both control and treated samples of DMF. The GC-MS data revealed that the isotopic abundance ratio of (PM+1)/PM in DMF was slightly decreased by 5.76% in T1, and increased by 48.73%, and 30.17% in T2, and T3 samples, respectively as compared to the control [where, PM- primary molecule, (PM+1)- isotopic molecule either for 13C or 2H or 15N]. Similarly, the isotopic abundance ratio of (PM+2)/PM was decreased by 10.34% in T1 and then increased upto 43.67% (T2) as compared to the control [where, (PM+2)- isotopic molecule for 18O]. In high performance liquid chromatography (HPLC), the treated DMF showed similar retention time (TR) as compared to the control with an additional small peak at 2.26 min appeared in the treated sample. In DSC thermogram the heat change in a sharp endothermic transition at around 61°C of treated DMF was increased by 152.56% as compared to the control. Further, C=O and C-N stretching frequencies of treated sample were shifted by 7 cm-1 and 3 cm-1, respectively towards low energy region in Fourier transform infrared (FT-IR) spectroscopy. These results suggested that biofield energy treatment has significantly altered the physical and spectroscopic properties of DMF, which could make them more stable solvent in organic synthesis and as a suitable formulation agent in polymer/paint industry.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

Viral load quantification is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of AIDS. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield treatment on HIV-1 and HCMV in terms of viral loads as surrogate marker. The viral load assay was performed on stored stock cultures of HIV infected human plasma samples before and after 7 days of biofield treatment using Roche COBAS® AMPLICOR analyzer. Viral load (HIV-1 RNA and HCMV DNAaemia) was considered as surrogate marker for assessment of the impact of Mr. Trivedi’s biofield treatment in HIV infected stored plasma samples. The viral load quantification of HIV-1 RNA in infected stored plasma samples was significantly reduced by 65% in biofield treated group as compared to control. Additionally, viral load of HCMV DNAaemia in infected stored plasma samples was also reduced by 80% in the biofield treated group as compared to control. Because, children are more prone to HCMV infection and adults are generally liable to suffer from HIV-1 infection. As the biofield treatment has reduced HCMV DNAaemia, it could be beneficial for HIV infected children populations. Altogether, data suggest that biofield treatment has significantly reduced the viral load quantification in HIV-1 and HCMV infected stored plasma samples and could be a suitable alternative treatment strategy for AIDS patients in near future.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 162 after revival (Study I). The revived sample of Gr. IIB was retreated on day 162 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively after their sub-culturing. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The antimicrobial susceptibility and minimum inhibitory concentration were significantly improved by 71.43%, out of twenty-eight and 56.25%, out of thirty-two, respectively in the treated cells of P. rettgeri as compared to the control. The biochemical reactions also showed the significant (60.61%) alteration in the treated sample with respect to control. The biotype numbers were substantially changed in all the treated groups as compared to the control. Moreover, the organism was changed as Proteus mirabilis in all the treated groups except in Gr. IIA, as compared to the control. These results suggested that biofield treatment has a significant impact on P. rettgeri in lyophilized as well as revived state.