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Gopal Nayak
Calcutta University
Position
Researcher
Department
Field of research
Natural Sciences (Biology)
Email
gopal@trivedisrl.com
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In Vitro Evaluation of Antifungal Sensitivity Assay of Biofield Energy Treated Fungi
Natural Sciences (Biology)
520 views
Date of upload:
15.12.2016
Co-author:
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Khemraj Bairwa, Snehasis Jana
Abstract:
Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Treatment groups received the biofield treatment, and control group was remained as untreated. Mini-API ID32C strip employed for evaluation of antifungal sensitivity and minimum inhibitory concentration (MIC). The results showed that sensitivity of Candida albicans in T1 cells was changed against itraconazole from intermediate (I) to resistance (R) on day 10. The Candida kefyr exhibited a change in susceptibility against itraconazole in T2 cell from S→I, on day 10. Likewise, Candida krusei showed the alterations in sensitivity against two antifungal drugs: fluconazole from S→I (T1 on day 10) and itraconazole S→I (T1 and T2 on all assessment days). The Cryptococcus neoformans changed from S→I in T1 cell on day 5 and 10, against itraconazole. Sensitivity of Candida tropicalis was also altered from I→R against flucytosine (T1 and T2, on all assessment days). Similarly, Saccharomyces cerevisae altered from S→I (T1) and S→R (T2) on day 10. The MIC values of antifungal drugs were altered in the range of 2-8 folds, as compared to the control. Fungal identification data showed the significant changes in species similarity of few tested fungi as C. albicans changed from 91.9% to 98.5 and 99.9% in T1 and T2 cells, respectively on day 10. C. krusei was changed from 97.9% to 85.9% (T2 day 10), and C. tropicalis was altered from 88.7% to 99.6% (T1 day 5) and 99.0% (T2). These findings suggest that biofield treatment could be applied to alter the susceptibility pattern of antifungal drug therapy in future.
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Effect of a Biofield Treatment on Plant Growth and Adaptation
Natural Sciences (Biology)
521 views
Date of upload:
15.12.2016
Co-author:
Nandini Altekar
Abstract:
Quantum mechanics was developed when human energies of consciousness were found to influence observations at the scale of elementary particles, here referred as non-contact biofield treat-ment or biofield energies. Quantum mechanics has also proved ef-ficacious in biological processes. The present experiments found an enhanced and significant impact of the biofield treatment on adaptive micropropagation response and callus induction of two plant spe-cies, Withania somnifera and Amaranthus dubius. The enhancement was perhaps due to greater focus on adaptation rather than specific mechanisms, showing high potential including at biochemical and genetic levels. Possible reasons for the enhancement are discussed and a possible model is presented, consistent with current scientific theory.
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Impact of Biofield Treatment on Growth and Anatomical Characteristics of Pogostemon cablin (Benth.)
Natural Sciences (Biology)
520 views
Date of upload:
15.12.2016
Co-author:
Shrikant Patil, Siddhivinayak Barve, Rashmi Tembe, Rummana Khan
Abstract:
Pogostemon cablin is a known aromatic plant which is cultivated for its essential oil widely applicated in perfumery and cosmetic industries. In the present study, the effect of biofield treatment was studied on the growth of P. cablin. For this study an in vitro culture system was set up in two groups, viz., control and treatment, each of which was derived from three different explant sources, namely leaf, node and petiole. Further these in vitro plantlets were hardened and transferred to external environment. The stomatal cells and epidermal hair growth were also studied at various morphogenetic stages. The study revealed that a single spell of biofield energy treatment produced significant increase in growth in treated group throughout all the morphogenetic phases from in vitro to in vivo level. A remarkable increase in stomatal cells and epidermal hair was also seen in treated group.
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Characterization of Physicochemical and Thermal Properties of Biofield Treated Ethyl Cellulose and Methyl Cellulose
Natural Sciences (Analytical Chemistry, Method Development (Chemistr)
472 views
Date of upload:
16.12.2016
Co-author:
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Rakesh Kumar Mishra, Snehasis Jana
Abstract:
Cellulose and its derivatives are used as potential matrices for biomaterials and tissue engineering applications. The objective of present research was to investigate the influence of biofield treatment on physical, chemical and thermal properties of ethyl cellulose (EC) and methyl cellulose (MC). The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The biofield treated polymers are characterized by Fourier transform infrared spectroscopy (FT-IR), CHNSO analysis, X-ray diffraction study (XRD), Differential Scanning calorimetry (DSC), and thermogravimetric analysis (TGA). FT-IR analysis of treated EC showed downward shifting in C-O-C stretching peak from 1091→1066 cm-1 with respect to control. However, the treated MC showed upward shifting of –OH stretching (3413→3475) and downward shifting in C-O stretching (1647→1635 cm-1) vibrations with respect to control MC. CHNSO analysis showed substantial increase in percent hydrogen and oxygen in treated polymers with respect to control. XRD diffractogram of EC and MC affirmed the typical semi-crystalline nature. The crystallite size was substantially increased by 20.54% in treated EC with respect to control. However, the treated MC showed decrease in crystallite by 61.59% with respect to control. DSC analysis of treated EC showed minimal changes in crystallization temperature with respect to control sample. However, the treated and control MC did not show any crystallization temperature in the samples. TGA analysis of treated EC showed increase in thermal stability with respect to control. However, the TGA thermogram of treated MC showed reduction in thermal stability as compared to control. Overall, the result showed substantial alteration in physical, chemical and thermal properties of treated EC and MC.

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