Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Rakesh Kumar Mishra, Snehasis Jana

2-Aminopyridine is an important compound, which is used as intermediate for the synthesis of pharmaceutical compounds. The present work was aimed to assess the effect of Mr. Trivedi’s biofield energy treatment on the physical, thermal and spectral characteristics of 2-AP. The work was accomplished by dividing the sample in two parts i.e. one part was remained untreated, and another part had received biofield energy treatment. Subsequently, the samples were analyzed using various characterization techniques such as X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, ultra violet-visible spectroscopy, and Fourier transform infrared spectroscopy. The XRD analysis revealed a decrease in crystallite size of the treated sample (91.80 nm) as compared to the control sample (97.99 nm). Additionally, the result showed an increase in Bragg’s angle (2θ) of the treated sample as compared to the control. The DSC and Differential thermal analysis analysis showed an increase in melting temperature of the treated 2-AP with respect to the control. Moreover, the latent heat of fusion of the treated sample was increased by 3.08%. The TGA analysis showed an increase in onset of thermal degradation (Tonset), and maximum thermal decomposition temperature (Tmax) of the treated 2-AP as compared to the control sample. Additionally, the treated sample showed a reduction in weight loss as compared with the control indicating higher thermal stability of the sample. UV-visible analysis showed no changes in the absorption peak of the treated sample as compared to the control. The FT-IR spectroscopic results showed downward shifting of C-H stretching vibration 2991→2955 cm-1 in treated sample with respect to the control.

Mahendra Kumar Trivedi, Shrikant Patil, Rama Mohan Tallapragada, Rakesh Mishra

Cellulose being an excellent biopolymer has cemented its place firmly in many industries as a coating material, textile, composites, and biomaterial applications. In the present study, we have investigated the effect of biofield treatment on physicochemical properties of cellulose and cellulose acetate. The cellulose and cellulose acetate were exposed to biofield and further the chemical and thermal properties were investigated. X-ray diffraction study asserted that the biofield treatment did affect the crystalline nature of cellulose. The percentage of crystallite size was found increased significantly in treated cellulose by 159.83%, as compared to control sample. This showed that biofield treatment was changing the crystalline nature of treated cellulose. However treated cellulose acetate showed a reduction in crystallite size (-17.38%) as compared to control sample. Differential Scanning Calorimetry (DSC) of treated cellulose showed no improvement in melting temperature as compared to control sample. Contrarily cellulose acetate showed significant improvement in melting temperature peak at 351.91ºC as compared to control (344ºC) polymer. Moreover percentage change in latent heat of fusion (∆H) was calculated from the DSC thermogram of both treated and control polymers. A significant increase in percentage ∆H of both treated cellulose (59.09%) and cellulose acetate (105.79%) polymers indicated that biofield treatment enhanced the thermal stability of the treated polymers. CHNSO analysis revealed a significant change in percentage hydrogen and oxygen of treated cellulose (%H-17.77, %O-16.89) and cellulose acetate (%H-5.67, %O-13.41). Though minimal change was observed in carbon percentage of both treated cellulose (0.29%) and cellulose acetate (0.39%) polymers as compared to their respective control samples. Thermo gravimetric analysis and Differential thermo gravimetric (TGA-DTG) analysis of treated cellulose acetate (353ºC) showed increased maximum thermal decomposition temperature as compared to control polymer (351ºC). This showed the higher thermal stability of the treated cellulose acetate polymer; although the maximum thermal decomposition temperature of treated cellulose (248ºC) was decreased as compared to control cellulose (321ºC). These outcomes confirmed that biofield treatment has changed the physicochemical properties of the cellulose polymers.

Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Omprakash Latiyal, Snehasis Jana

Lithium has gained extensive attention in medical science due to mood stabilizing activity. The objective of the present study was to evaluate the impact of biofield treatment on physical, atomic, and thermal properties of lithium powder. The lithium powder was divided into two parts i.e., control and treatment. Control part was remained as untreated and treatment part received Mr. Trivedi’s biofield treatment. Subsequently, control and treated lithium powder samples were characterized using X-ray diffraction (XRD), Differential scanning calorimetry (DSC), Thermogravimetric analysis-differential thermal analysis (TGA-DTA), Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). XRD data showed that lattice parameter, unit cell volume, density, atomic weight, and nuclear charge per unit volume of lithium were altered after biofield treatment. The crystallite size of treated lithium was increased by 75% as compared to control. DSC analysis exhibited an increase in melting temperature of treated lithium powder upto 11.2% as compared to control. TGA-DTA analysis result showed that oxidation temperature, which found after melting point, was reduced upto 285.21°C in treated lithium as compared to control (358.96°C). Besides, SEM images of control and treated lithium samples showed the agglomerated micro particles. Moreover, FT-IR analysis data showed an alteration in absorption band (416→449 cm-1) in treated lithium sample after biofield treatment as compared to control. Overall, data suggested that biofield treatment has significantly altered the physical, atomic, and thermal properties of lithium powder.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. The lyophilized strain of V. parahaemolyticus was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, whereas, Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment of Gr. IIB on day 142 (Study II), the sample was divided into three separate tubes. The tubes first, second and third were analyzed on day 5, 10, and 15, respectively. The biochemical reaction and biotyping were performed using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of V. parahaemolyticus with other bacterial species after the treatment. The results of biochemical reactions were altered 24.24%, out of thirty-three in the treated groups with respect to the control. Moreover, negative (-) reaction of urea was changed to positive (+) in the revived treated Gr. IIB, Study II on day 15 as compared to the control. Besides, biotype number was substantially changed in all the treated groups as compared to the control. However, change in organisms were reported in Gr. IIA on day 10 and in Gr. IIB; Study II on day 5 as Shewanella putrefaciens and Moraxella/Psychrobacter spp., respectively with respect to the control i.e. Vibrio sp. SF. 16S rDNA analysis showed that the identified sample in this experiment was V. parahaemolyticus after biofield treatment, and the nearest homolog genus-species was observed as Vibrio natriegens with 98% gene identity. The results envisaged that the biofield energy treatment showed an alteration in biochemical reaction pattern and biotype number on the strain of V. parahaemolyticus.