Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.

Bonnie P. Hegarty-Diaz, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

The objective of this study was to evaluate the impact of The Trivedi Effect®- Consciousness Energy Healing Treatment based herbomineral formulation on various skin parameters using HFF-1, HaCaT, and B16-F1 cell lines. The test formulation was composed of minerals (zinc, selenium, and molybdenum), and L-ascorbic acid along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). Test formulation and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment by Bonnie P. Hegarty-Diaz and denoted as treated, while other part was coded as untreated groups. MTT assay result showed that the test formulation was found safe and nontoxic at tested concentration 40 µg/mL. Fibroblast cell proliferation assay showed significant increased cell proliferation by 26.83% at 8.75 µg/mL in BT-DMEM + BT-Test formulation group compared to the untreated group. The level of collagen was significantly increased by 22.05% and 22.24% (at 2.5 µg/mL) in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively. However, collagen amount was increased by 11.08% and 9.52% in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL, compared to untreated group. Similarly, elastin synthesis was increased in the BT-DMEM + UT-Test formulation group by 19.98%, 72.54% and 105.04% at the concentrations 10, 5, and 2.5 µg/mL, respectively compared to the untreated group. However, level of hyaluronic acid was increased by 5.19% at 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with the untreated group. Besides, melanin synthesis inhibition was found to be inhibited by 14.80% and 17.40% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed improve cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 10.69%, 6.9%3, and 11.86%, respectively at 0.625 µg/mL compared with the untreated group. In addition, wound healing scratch assay data suggest significantly higher cellular migration of fibroblast and keratinocytes cells in HFF-1 and HaCaT cells lines, respectively. In conclusion, Biofield Energy Healing (The Trivedi Effect®) based test formulation and DMEM could be beneficial against various skin disorders such psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections and can be significantly used as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action.

Deborah Lea Smith, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

The aim of this study was to evaluate the effect of the Consciousness Energy Healing based test formulation and cell medium (DMEM) against various skin health parameters. The test formulation and DMEM were divided into two parts. One part received the Consciousness Energy Healing Treatment (The Trivedi Effect®) by Deborah Lea Smith and termed as the Biofield Energy Treated sample, while other part denoted as untreated test item. The study was assessed for the measurement of collagen, elastin, hyaluronic acid, melanin synthesis, cell viability against UV-B induced stress, and wound healing activity. MTT assay showed that the test formulation was safe and nontoxic in three cell lines. Bromodeoxyuridine (BrdU) assay data showed that the percent cell proliferation was increased significantly by 22.69% and 34.99% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen was increased by 15.12% and 15.26% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. Elastin was significantly increased by 9.10%, 13.71%, and 12.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL compared to the untreated group. Elastin was also increased significantly by 17.78% and 6.01% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was significantly increased by 3.90%, 5.37%, and 6.82% in the UT-DMEM + BT-Test formulation at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Melanin level was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection was significantly increased by 21.32%, 23.81%, and 7.20% at 0.625, 1.25, and 2.5 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared to the untreated group. Wound healing assay exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, the Biofield Energy Treated test formulation and DMEM (The Trivedi Effect®) were found to be safe and can be developed as an effective cosmetic product to protect and treat the skin problems including infection, photosensitivity, acne, hives, chickenpox, eczema, rosacea, seborrheic dermatitis, psoriasis, erythema, contact dermatitis, skin aging, wrinkles, etc.

Johanne Dodon, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

The aim of the study was to evaluate the effect of the The Trivedi Effect®- Consciousness Energy Healing Treatment based new proprietary herbomineral formulation and cell medium (DMEM) on various skin health parameters using HFF-1, HaCaT, and B16-F10 cell lines. It contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts each, one part of each was coded as untreated (UT), while the second part of each was treated with the Biofield Treatment by Johanne Dodon and denoted as the Biofield Energy Treated (BT) test items. MTT assay result showed that the test formulation was safe and nontoxic up to 80 µg/mL concentration. The fibroblast cell proliferation assay using BrdU method showed a significant increased cell proliferation at 2.13, 8.75, and 17.53 µg/mL by 3.90%, 14.68% and 29.54%, respectively in the BT-DMEM + BT-Test formulation group compared to the untreated group. The collagen data showed an increased synthesis by 7.42% and 3.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Similarly, the elastin synthesis was increased by 19.8% and 25.8% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL with respect to the untreated group. However, the Biofield Energy Treated test formulation showed an increased level of hyaluronic acid by 1.7% in UT-DMEM + BT-Test formulation group compared to the untreated group. Melanin synthesis inhibition was observed by 15.21% and 11.43% in UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation groups, respectively at 0.125 µg/mL compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling effects showed an improved cell viability by 7.65% at 1.25 µg/mL in the BT-DMEM + BT-Test formulation experimental group compared with the untreated test group. In addition, the Biofield Energy Treated Test formulation/DMEM showed a significant wound healing activity and high cellular migration of fibroblast and keratinocytes in HFF-1 and HaCaT cells, respectively. Overall, the data suggested that Biofield Treated formulation/DMEM could be helpful for skin healing, improvement the skin health, elasticity, and tightness, as well as, improving skin health issues such as wrinkling, pigmentation disorders, redness, swelling, skin cancer, aging, rashes, and skin sagging.