Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The LC-MS analysis of the control and treated samples showed a very close retention time (Rt), indicated that the polarity of the phytoconstituents present in the root extract are same. The numbers of peaks observed in the total ion chromatograms were 28 and 29 in the control and treated samples, respectively. The change in the peak height% of the phytoconstituents in the treated sample was altered significantly within the range of -50.91% to 118.12% compared with the control sample. Similarly, the change in the peak area% of most of the phytoconstituents in the treated ashwagandha was significantly altered within the range of -54.95% to 66.95% compared with the control sample. An additional peak was appeared in the treated sample at Rt of 5.72 minutes, which was not found in the control sample. The LC-MS spectra indicated the presence of possible withanolides like -hydroxy-2,3-dihydro-withanolide F, withanolide A, withaferine A, withanone, withanolide D, ixocarpalactone A, withanolide S, thiowithanolide, etc. in both the samples. The peak are percentage (%) was altered in the identified withanolides, but withanolide sulfoxide was increased significantly by 12.44% in the treated sample compared with the control sample. These results indicated that The Trivedi Effect® - Biofield Energy Treatment might have an impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract. This could be the probable cause of alteration in the peak height, peak area, and appearance of a new peak in the treated sample. As a result, the concentrations of the phytoconstituents altered in the treated sample compared with the control sample. The treated ashwagandha root extract would be helpful for designing better pharmaceutical/nutraceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, ulcer, immunological disorders, stress, arthritis, etc.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Sodium selenate was considered for the current study, which is an important nutrient used for the prevention and treatment of cancer, inflammatory diseases, diabetes, etc. The objective of the current study was to examine the impact of The Trivedi Effect® - Consciousness Energy Healing Treatment (Biofield Energy Healing treatment) on the physico-chemical, thermal, and behavioral properties of sodium selenate using PXRD, PSD, FT-IR, UV-vis, TGA, and DSC analysis. Sodium selenate sample was divided into two parts – one part was control, while another part was treated with the Biofield Energy remotely by twenty renowned Biofield Energy Healers and termed as the treated sample. The PXRD analysis showed that the crystallite size of the treated sample significantly altered in the range of -50.01% to 59.97% compared to the control sample. The average crystallite size of the treated sample was significantly increased by 7.32% compared with the control sample. The PSD analysis indicated that the particle size of the treated sample at d10, d50, and d90 values were increased by 6.84%, 8.04%, and 3.04%, respectively compared with the control sample. Thus, the surface area of the Biofield Energy Treated sample was significantly decreased by 7.41% compared with the control sample. The FT-IR spectra indicated the presence of sharp and strong absorption bands at 887 and 888 cm-1 due to the Se=O stretching in the control and treated sample, respectively. The wavelength of maximum absorbance from the UV-vis spectroscopic analysis of the Biofield Energy Treated sample (205.2 nm) was increased by 0.39% compared to the control sample (204.4 nm). The TGA analysis revealed the total weight loss of the Biofield Energy Treated sample was significantly reduced by 12.63% compared with the control sample. The DSC analysis showed that the vaporization temperature of the Biofield Energy Treated sample (95.14°C) was increased compared to the control sample (94.28°C). But, the latent heat of vaporization was decreased significantly in the treated sample by 43.67% compared to the control sample. Thus, The Trivedi Effect® - Consciousness Energy Healing Treatment might lead to generate a new polymorphic form of sodium selenate, which would have better powder flowability and long-term storage stability compared with the control sample. The Biofield Energy Treated sodium selenate would be helpful for designing better nutraceutical/pharmaceutical formulations against immunological disorders, inflammatory diseases, aging, stress, infectious diseases, Alzheimer’s disease, cancer, diabetes, heart diseases, etc.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

Zinc chloride is an important inorganic compound used as a source of zinc in various pharmaceutical/nutraceutical formulations. The objective of the current study was to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physical, structural, thermal, and behavioral properties of zinc chloride using PXRD, PSD, FT-IR, UV-vis, and DSC analysis. Zinc chloride was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. A significant alteration of the crystallite size and relative intensities of the PXRD peaks was observed in The Trivedi Effect® treated sample compared with the control sample. The average crystallite size of the treated sample was significantly decreased by 16.31% compared with the control sample. The particle size values at d10, d50, and d90 were significantly decreased by 11.54%, 7.75%, and 1.34%, respectively in the treated sample compared with the control sample. Therefore, the surface area of the treated sample was significantly increased by 8.36% compared with the control sample. The FT-IR spectroscopic analysis revealed that Zn-Cl stretching in both the control and treated samples were found at 516 cm-1. The UV-vis analysis exhibited that wavelength of the maximum absorbance (λmax) of the control and treated samples were at 196.6 nm and 196.4 nm, respectively. The DSC analysis exhibited that the melting and decomposition temperature were increased by 1.25% and 0.21%, respectively in the treated zinc chloride compared to the control sample. The latent heat of fusion of the treated sample (434.97 J/g) was significantly increased by 84.74% compared with the control sample (235.45 J/g). Similarly, the enthalpy of decomposition of the treated sample (1241.86 J/g) was significantly increased by 307.21% compared with the control sample (304.97 J/g). So, the treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. The current study anticipated that The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to produce a thermally more stable new polymorphic form of zinc chloride, which would be more soluble and bioavailable compared with the untreated compound. Hence, the treated zinc chloride would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, aging, stress, cancer, etc.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana

The present study was aimed to evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) and PM+2/PM (18O/16O or 26Mg/24Mg) were significantly decreased in the treated sample by 35.97% and 66.77%, respectively compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly reduced compared with the control sample. Thus, The Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.