William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.

Jagdish Singh, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

Oxidative stress causes serious skin damage that is characterized by ageing, wrinkling, roughness, laxity and pigmentation. In the present work, the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the herbomineral test formulation and cell medium (DMEM) was evaluated for skin health parameters. The test formulation was consisted of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by Jagdish Singh and was termed as the Biofield Treated (BT) sample, while other was denoted as the untreated (UT) samples. MTT assay showed that test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 149.13% and 118.80% at 8.75 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 10.38%, 14.66%, and 48.24% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + UT-Test formulation group, compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 5.93%, 1.43%, and 1.43% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.0625 µg/mL compared with the untreated group. However, melanin synthesis was decreased by 11.71% and 15.75% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively. Anti-wrinkling effects exhibited improved cell viability by 17.19% and 28.68% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay showed a significantly improved healing rate upto 5% in the HFF-1 and HaCaT cells lines in the Biofield Energy Healing based test formulation. Overall, the data suggests that The Trivedi Effect® treated test formulation and DMEM has the capacity to improve the skin health and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.

James Jeffery Peoples, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

Currently in today’s world, herbomineral based cosmetic formulations are continuously gaining popularity globally because they have a high safety profile and skin protection effects. The aim of this study was to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against different skin health parameters. The test formulation and DMEM were divided into two parts. One part of each received the Consciousness Energy Healing Treatment by James Jeffery Peoples and were termed as the Biofield Energy Treated samples, while the other samples were denoted as the untreated test items. MTT assay showed more than 70% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was increased by 251.30%, 373.36%, and 722.48% at 8.75, 17.5, and 35 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Collagen synthesis was significantly (p≤0.001) increased by 32.96% in the UT-DMEM + BT-Test formulation group at 2.5 µg/mL with respect to the untreated group. Elastin was significantly (p≤0.001) increased by 69.49% and 17.13% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. The level of hyaluronic acid was increased remarkably by 14.78% in the BT-DMEM + UT-Test formulation group at 1.25 µg/mL compared to the untreated group. Melanin was suppressed significantly by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection against UV-B exposure revealed that the percent cell viability was increased by 4.23% in the BT-DMEM + BT-Test formulation group at 2.5 µg/mL compared to the untreated group. The wound healing activity by scratch assay showed significant wound closure and cell migration activity in the test formulation and DMEM group in both HFF-1 and HaCaT cells compared to the untreated group. Altogether, the data suggests that the Biofield Energy Treated DMEM and test formulation showed better responses compared to the untreated group. Overall, the Biofield Energy Treated test formulation could be developed as an effective cosmetic product to protect and treat various skin problems including infection, photosensitivity, cutis rhomboidalis nuchae, erythema, contact dermatitis, behcet's disease, blepharitis, boil, bowen's disease, skin aging, wrinkles and/or change in skin colour, etc.

Janice Patricia Kinney, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

The aim of the present study was to evaluate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against various skin health parameters using three cell lines i.e., HFF-1, HaCaT, and B16-F10. The various study parameters viz. collagen, elastin, hyaluronic acid, melanin, cell viability against UV-B induced stress, and wound healing were evaluated. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Janice Patricia Kinney and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 70% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The collagen synthesis was significantly (p≤0.001) increased by 22.42% and 17.48% in the UT-DMEM + BT-Test formulation group at 0.63 and 2.5 µg/mL, respectively in relation to the UT-DMEM + UT-Test formulation group. The Elastin level was significantly (p≤0.001) increased by 28.41% in the BT-DMEM + BT-Test formulation group at 5 µg/mL compared to the untreated group. Hyaluronic acid at 0.63 µg/mL was increased significantly by 15.90% in the BT-DMEM + BT-Test formulation group compared to the untreated group. The level of melanin was reduced significantly by 9.25% and 7.26% in the BT-DMEM + BT-Test formulation group at 0.013 and 0.063 µg/mL, respectively in relation to the untreated group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased significantly by 17.88%, 20.10%, and 25.77% in the BT-DMEM + BT-Test formulation group at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 and HaCaT cells compared to the UT-DMEM + UT-Test formulation. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better responses compared to the untreated medium and test formulation with respect to the tested skin health parameters. Therefore, the Biofield Energy Healing and the Treated test formulation could be developed as an effective cosmetic product to protect and treat the various skin problems including infection, photosensitivity, erythema, contact dermatitis, seborrheic dermatitis, athlete's foot, psoriasis, erythema, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color, etc.