Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00% respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant (35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantially changed in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers were changed in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that the identified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, the nearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These results suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes. First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treated cells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant to susceptible after biofield treatment, with support of decreased MIC value (>8 to ≤ 4 µg/mL) by two-fold in all the treated samples as compared to the control. Biochemical reactions also showed significant (42.42%) alteration in the treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA (53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus) on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with species were substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and (53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showed that the identified microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. The nearest homolog genus-species of C. braakii was found to be Citrobacter werkmanii (Accession No. AF025373). The results suggested that biofield treatment has a significant impact on C. braakii in lyophilized as well as revived state.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Khemraj Bairwa, Snehasis Jana

Para nitroaniline (p-Nitroaniline) is an organic compound, used as an intermediate in the synthesis of pharmaceuticals drugs, gasoline and dyes. The present study was attempted to investigate the influence of biofield treatment on p-nitroaniline. The study was performed in two groups i.e., control and treatment. The treatment group was subjected to Mr. Trivedi’s biofield treatment. The control and treated samples of p-nitroaniline were characterized using Fourier transform infrared (FT-IR) spectroscopy, Ultraviolet-visible (UV-Vis) spectroscopy, high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and differential scanning calorimetry (DSC). FT-IR spectral analysis result suggested the alteration in wavenumber of some groups with respect to control. For instance, the C=C and C-C stretching were observed at 1570 cm-1 and 1430 cm-1, respectively in control sample that were shifted to 1585 cm-1 and 1445 cm-1, respectively after biofield treatment. UV spectral analysis revealed the similar pattern of absorbance maxima (λmax) in both control and treated samples. HPLC data showed an alteration in the retention time of p-nitroaniline peak in treated sample (3.25 min) with respect to control (2.75 min). GC-MS results showed a significant change in the isotopic abundance (δ) of 13C and 18O in treated sample as compared to control. DSC data showed that latent heat of fusion (∆H) of treated p-nitroaniline was substantially decreased by 10.66% as compared to control. However, the melting point remained same in both control and treated sample of p-nitroaniline. Overall, results obtained from different analytical techniques such as FT-IR, HPLC, GC-MS, and DSC suggested that biofield treatment has significant impact on spectral, physical and thermal properties of p-nitroaniline with respect to control sample.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Khemraj Bairwa, Snehasis Jana

Methyl-2-naphthyl ether (MNE) is an organic compound and used as the primary moiety for the synthesis of several antimicrobial and anti-inflammatory agents. This study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of MNE. The study was carried out in two groups i.e., control and treated. The treated group was subjected to Mr. Trivedi’s biofield treatment. Afterward, the control and treated samples of MNE were evaluated using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric analysis-derivative thermogravimetric analysis (TGA-DTG), Fourier transform infrared (FT-IR), and ultraviolet-visible (UV-Vis) spectroscopy. The XRD study exhibited the decrease in average crystallite size by 30.70%. The surface area analysis showed 5.32% decrease in surface area of the treated sample with respect to the control. The DSC thermogram of treated MNE exhibited no significant change in the melting temperature; however, the latent heat of fusion was slightly increased (0.83%) after biofield treatment as compared to the control sample. The TGA analysis showed the onset temperature of thermal degradation at 158℃ in the control sample that was reduced to 124℃ after biofield treatment. The result showed about 21.52% decrease in onset temperature of thermal degradation of treated MNE as compared to the control. Similarly, the end-set temperature of thermal degradation was also reduced by 13.51% after biofield treatment with respect to the control. The FT-IR and UV spectroscopic studies did not show any changes in the wavenumber and wavelength, respectively in treated MNE with respect to the control. Overall, the XRD, surface area and thermal analysis suggest that biofield treatment has the impact on physical and thermal properties of the treated MNE as compared to the control.