Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Charan Mondal, Snehasis Jana

Among the oilseeds grown around the world, mustard is one of the important crop worldwide due to its wide adaptability and high yielding capacity. Owing to the importance of its utilities as condiment, cooking oil and some medical aids, the demand for its seed production is too high. The present study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on mustard (Brassica juncea) for its growth-germination of seedling, glutathione (GSH) content in leaves, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of B. juncea was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi’s biofield energy treatment and referred as the treated sample. The growth-germination of B. juncea seedling data exhibited that the biofield treated seeds were germinated faster on day 5 as compared to the control (on day between 7-10). The shoot and root length of seedling were slightly increased in the treated seeds of 10 days old with respect to untreated seedling. Moreover, the major plant antioxidant i.e. GSH content in mustard leaves was significantly increased by 206.72% (p<0.001) as compared to the untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was increased by 15.81% and 12.99%, respectively with respect to the control. Besides, the DNA fingerprinting data using RAPD revealed that the treated sample showed an average 26% of DNA polymorphism as compared to the control. The overall results envisaged that the biofield energy treatment on mustard seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the biofield energy treatment of mustard seeds could be used as an alternative way to increase the production of mustard.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Parthasarathi Panda, Snehasis Jana

The stable isotope ratio analysis is widely used in several scientific fields such as agricultural, food authenticity, biochemistry, metabolism, medical research, etc. Resorcinol is one of the most versatile chemicals used for the synthesis of several pharmaceuticals, dyes, polymers, organic compounds, etc. The current research work was designed to investigate the impact of the biofield energy treatment on the isotopic abundance ratios of 13C/12C or 2H/1H or 17O/16O (PM+1/PM) and 18O/16O (PM+2/PM) in resorcinol using Gas chromatograph - mass spectrometry (GC-MS) technique. Resorcinol was divided into two parts - one part was control and another part was considered as biofield energy treated sample. The biofield energy treatment was accomplished through unique biofield energy transmission by Mr. Mahendra Kumar Trivedi (also called as The Trivedi Effect®). T1, T2, T3, and T4 were denoted by different time interval analysis of the biofield treated resorcinol in order to understand the influence of the biofield energy treatment on isotopic abundance ratio with respect to the time. The GC-MS spectra of the both control and biofield treated resorcinol exhibited the presence of molecular ion peak [M+] at m/z 110 (calculated 110.04 for C6H6O2) along with major fragmented peaks at m/z 82, 81, 69, 53, and 39. The relative peak intensities of the fragmented ions in biofield treated resorcinol (particularly T2) was significantly changed with respect to the control sample. The stable isotope ratio analysis in resorcinol using GC-MS revealed that the percentage change of the isotopic abundance ratio of PM+1/PM was increased in the biofield treated resorcinol at T1, T2, T3 and T4 by 1.77%, 165.73%, 0.74%, and 6.79%, respectively with respect to the control sample. Consequently, the isotopic abundance ratio of PM+2/PM in the biofield treated resorcinol at T2, T3, and T4 were enhanced by 170.77%, 3.08%, and 12.31%, respectively with respect to the control sample. Briefly, 13C, 2H, 17O contributions from (C6H6O2)+ to m/z 111 and 18O contribution from (C6H6O2)+ to m/z 112 for the biofield treated resorcinol at T2 and T4 were significantly altered as compared to the control sample. For this reasons, biofield treated resorcinol might exhibit altered physicochemical properties like diffusion velocity, mobility and evaporation rate, reaction rate, binding energy, and stability. Biofield treated resorcinol could be valuable in pharmaceutical and chemical industries as intermediates during the preparation of pharmaceuticals and chemical compounds by altering its physicochemical properties, the reaction rate and selectivity, the study of the reaction mechanism and facilitating in designing extremely effective and specific enzyme inhibitors.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Parthasarathi Panda, Snehasis Jana

Thymol is a natural monoterpenoid phenol possessing various pharmacological activities such as antimicrobial, antioxidant, etc. The stable isotope ratio analysis has drawn attention in numerous fields such as agricultural, food authenticity, biochemistry, metabolism, medical research, etc. An investigation of the effect of the biofield energy treatment (The Trivedi Effect®) on the isotopic abundance ratios of PM+1/PM and PM+2/PM in thymol using gas chromatography - mass spectrometry was attempted in this study. The sample, thymol was divided into two parts - one part was denoted as control and another part was referred as biofield energy treated sample that was given Mr. Trivediꞌs unique biofield energy. T1, T2, T3, and T4 were represented to different time interval analysis of the biofield treated thymol. The GC-MS spectra of the both control and biofield treated thymol indicated the presence of molecular ion peak [M+] at m/z 150 (calculated 150.10 for C10H14O) along with the similar pattern of fragmentation. The relative intensities of the parent molecule and other fragmented ions of the biofield treated thymol were enhanced as compared to the control thymol. The percentage change of the isotopic abundance ratio of PM+1/PM in the biofield treated thymol at T1, T2, T3 and T4 was increased by 3.25, 6.31, 96.75, and 140.25%, respectively as compared to the control thymol. In addition, the percentage change of the isotopic abundance ratio of PM+2/PM was increased in the biofield treated thymol at T1, T2, T3, and T4 by 5.33, 8.00, 101.33, and 140.00%, respectively with respect to the control sample. In summary, 13C, 2H, and 17O contributions from (C10H14O)+ to m/z 151 and 18O contribution from (C10H14O)+ to m/z 152 in the biofield treated thymol were significantly increased gradually with respect to the time and was found that biofield energy treatment has time dependent effect on it. Hence, the biofield energy treated thymol might display altered isotope effects such as physicochemical and thermal properties, binding energy and the reaction kinetics with respect to the control sample. So, biofield energy treated thymol could be advantageous for designing the synthetic scheme for the preparation of pharmaceuticals through its kinetic isotope effects. Besides, biofield treated thymol might be useful to overcome the problems associated with thymol for e.g. pungent flavor, high dose requirement for the activity through understanding its isotope effects and the determination of its pharmacokinetic profile, bioavailability.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Kalyan Kumar Sethi, Snehasis Jana

Nitrophenols are the synthetic organic chemicals used for the preparation of synthetic intermediates, organophosphorus pesticides, and pharmaceuticals. The objective of the present study was to evaluate the effect of biofield energy treatment on the isotopic abundance ratios of PM+1/PM, and PM+2/PM in o- and m-nitrophenol using the gas chromatography-mass spectrometry. The o- and m-nitrophenol were divided into two parts - one part was control sample, and another part was considered as biofield energy treated sample, which received Mr. Trivedi’s biofield energy treatment (The Trivedi Effect®). The biofield energy treated nitrophenols having analyzed at different time intervals were designated as T1, T2, T3, and T4. The GC-MS analysis of both the control and biofield treated samples indicated the presence of the parent molecular ion peak of o- and m-nitrophenol (C6H5NO3+) at m/z 139 along with major fragmentation peaks at m/z 122, 109, 93, 81, 65, and 39. The relative peak intensities of the fragmented ions in the biofield treated o- and m-nitrophenol were notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis using GC-MS revealed that the isotopic abundance ratio of PM+1/PM in the biofield energy treated o-nitrophenol at T2 and T3 was significantly increased by 14.48 and 86.49%, respectively as compared to the control sample. Consequently, the isotopic abundance ratio of PM+2/PM in the biofield energy treated sample at T2 and T3 was increased by 11.36, and 82.95%, respectively as compared to the control sample. Similarly, in m-nitrophenol, the isotopic abundance ratio of PM+1/PM in the biofield energy treated sample at T1, T3, and T4 was increased by 5.82, 5.09, and 6.40%, respectively as compared to the control sample. Subsequently, the isotopic abundance ratio of PM+2/PM at T1, T2, T3 and T4 in the biofield energy treated m-nitrophenol was increased by 6.33, 3.80, 16.46, and 16.46%, respectively as compared to the control sample. Overall, the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O), and PM+2/PM (18O/16O) were altered in the biofield energy treated o- and m-nitrophenol as compared to the control increased in most of the cases. The biofield treated o- and m-nitrophenol that have improved isotopic abundance ratios might have altered the physicochemical properties and could be useful in pharmaceutical and chemical industries as an intermediate in the manufacturing of pharmaceuticals and other useful chemicals for the industrial application.