Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Rakesh Kumar Mishra, Snehasis Jana

Study background: 2,4-Dihydroxybenzophenone (DHBP) is an organic compound used for the synthesis of pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of DHBP. The study was performed in two groups (control and treated). The control group remained as untreated, and the treated group received Mr. Trivedi’s biofield energy treatment. Methods: The control and treated DHBP samples were further characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), laser particle size analyser, surface area analyser, Fourier transform infrared (FT-IR) spectroscopy, and ultra violet-visible spectroscopy (UV-vis) analysis. Results: The XRD study indicated a slight decrease in the volume of the unit cell and molecular weight of treated DHBP as compared to the control sample. However, XRD study revealed an increase in average crystallite size of the treated DHBP by 32.73% as compared to the control sample. The DSC characterization showed no significant change in the melting temperature of treated sample. The latent heat of fusion of the treated DHBP was substantially increased by 11.67% as compared to the control. However, TGA analysis showed a decrease in the maximum thermal decomposition temperature (Tmax) of the treated DHBP (257.66ºC) as compared to the control sample (260.93ºC). The particle size analysis showed a substantial increase in particle size (d50 and d99) of the treated DHBP by 41% and 15.8% as compared to the control sample. Additionally, the surface area analysis showed a decrease in surface area by 9.5% in the treated DHBP, which was supported by the particle size results. Nevertheless, FT-IR analysis showed a downward shift of methyl group stretch (2885→2835 cm-1) in the treated sample as compared to the control. The UV analysis showed a blue shift of absorption peak 323→318 nm in the treated sample (T1) as compared to the control. Conclusion: Altogether, the results showed significant changes in the physical, thermal and spectral properties of treated DHBP as compared to the control.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Study background: Multidrug resistant Escherichia coli (MDR E. coli) has become a major health concern, and failure of treatment leads to huge health burden. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on E. coli. Methods: Four MDR clinical lab isolates (LSs) of E. coli (LS 8, LS 9, LS 10, and LS 11) were taken and divided into two groups i.e. control and biofield treated. Control and treated samples were identified with respect to its antimicrobial sensitivity assay, biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with its respective control group. Results: Antimicrobial sensitivity assay showed 50% alteration in sensitivity of total tested antimicrobials in treated group of MDR E. coli isolates. MIC results showed the alteration in MIC of about 40.63% antimicrobials out of thirty two tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Ticarcillin/k-clavulanate showed improved sensitivity (R → I) with decreased MIC value in LS 9 as compared to control. A fourfold and twofold decreased in MIC values were reported in case of piperacillin/tazobactam (in LS 9) and chloramphenicol (in LS 8), respectively as compared to respective control. Biochemical study showed a 39.39% alteration in biochemical reactions after treatment among four isolates of E. coli as compared to control. A significant change in biotype numbers were reported in three clinical isolates (i.e. LS 8, LS 9, and LS 11) of MDR E. coli as compared to control. On the basis of changed biotype number (7774 5272) after biofield treatment, organism with maximum probability was identified as Enterobacter aerogenes in LS 8 as compared to control, (E. coli, 7711 5012). Conclusion: Overall results suggest that Mr Trivedi’s biofield treatment has a significant effect on altering the antimicrobial sensitivity, biochemical reactions and biotype number of MDR isolates of E. Coli.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gunin Saikia, Snehasis Jana

Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). BHT and 4-MP samples were divided into two parts: control and treated. The control group remained untreated while the treated group was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using GC-MS. The GC-MS data revealed that the isotopic abundance ratio of 13C/12C or 2H/1H (PM+1)/PM and 18O/16O (PM+2)/PM increased significantly in treated BHT and 4-MP (where PM- primary molecule, PM+1- isotopic molecule either for 13C or 2H and PM+2 is the isotopic molecule for 18O). The isotopic abundance ratio of (PM+1)/PM in the treated BHT and 4-MP was increased up to 181.27% and 380.73% respectively as compared to their respective control. Moreover, the isotopic abundance ratio of (PM+2)/PM in the treated BHT and 4-MP increased up to 185.99% and 355.33% respectively. GC-MS data suggests that the biofield treatment significantly increased the isotopic abundance of 2H, 13C and 18O in the treated BHT and 4-MP as compared to the control.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Khemraj Bairwa, Snehasis Jana

Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane. The study was performed in two groups i.e., control and treatment. The treatment group subjected to Mr. Trivedi’s biofield treatment. The control and treated groups of triphenylmethane samples were characterized using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR), ultraviolet-visible (UV-Vis) spectroscopy, and gas chromatography-mass spectrometry (GC-MS). XRD study revealed decreases in average crystallite size (14.22%) of treated triphenylmethane as compared to control sample. Surface area analysis showed a slight increase (0.42%) in surface area of treated sample with respect to control. DSC thermogram of treated triphenylmethane showed the slight increase in melting point and latent heat of fusion with respect to control. TGA analysis of control triphenylmethane showed weight loss by 45.99% and treated sample showed weight loss by 64.40%. The Tmax was also decreased by 7.17% in treated sample as compared to control. The FT-IR and UV spectroscopic result showed the similar pattern of spectra. The GC-MS analysis suggested a significant decrease in carbon isotopic abundance (expressed in δ13C, ‰) in treated sample (about 380 to 524‰) as compared to control. Based on these results, it is found that biofield treatment has the impact on physical, thermal and carbon isotopic abundance of treated triphenylmethane with respect to control.